Delivery of MiRNA-92a Inhibitor Using RP1-Linked Peptide Elicits Anti-Inflammatory Effects in an Acute Lung Injury Model
DC Field | Value | Language |
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dc.contributor.author | Zhuang, Chuanyu | - |
dc.contributor.author | Piao, Chunxian | - |
dc.contributor.author | Choi, Myoungjee | - |
dc.contributor.author | Ha, Junkyu | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-07-06T16:24:24Z | - |
dc.date.available | 2022-07-06T16:24:24Z | - |
dc.date.created | 2021-11-22 | - |
dc.date.issued | 2021-07 | - |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/141497 | - |
dc.description.abstract | Acute lung injury (ALI) is an inflammatory lung disease. miRNA-92a (miR92a) is induced in the lungs of ALI patients and mediates inflammatory reactions. In this study, a RP1-linked R3V6 (RP1R3V6) peptide was synthesized and evaluated as a carrier of anti-microRNA-92a oligonucleotide (AMO92a) into the lungs of an ALI animal model. In addition to the carrier function, the RP1-linked peptide can have anti-inflammatory effects in the lungs, since RP1 is an antagonist of the receptors for advanced glycation end-products (RAGEs). In a gel retardation assay, the RP1R3V6 peptide formed a spherical complex with AMO92a. In an in vitro delivery assay to L2 rat lung epithelial cells, RP1R3V6 had a lower AMO92a delivery efficiency than R3V6 and polyethyleneimine (PEI25k; 25 kDa). However, RP1R3V6 had an additional anti-inflammatory effect, reducing tumor necrosis factor-alpha (TNF-alpha) in lipopolysaccharide-activated macrophage cells. With the combined effects of AMO92a and RP1, the RP1R3V6/AM092a complex reduced the miR92a level more efficiently than did the R3V6/AMO92a and PEI25k/AMO92a complexes. The RP1R3V6/AM092a complex was administered into the lungs of ALI animals by intratracheal instillation. As a result, the expression of phosphatase and tensin homolog, a target of miR92a, was increased in the lungs. Furthermore, the RP1R3V6/AM092a complex decreased the TNF-alpha and interleukin-1 beta (IL-1 beta) levels more efficiently than did the PEI25k/AMO92a and R3V6/AMO92a complexes, decreasing the damage in the lungs. These results suggest that RP1R3V6 is a useful carrier of AMO92a and has anti-inflammatory effects in an ALI animal model. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.title | Delivery of MiRNA-92a Inhibitor Using RP1-Linked Peptide Elicits Anti-Inflammatory Effects in an Acute Lung Injury Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1166/jbn.2021.3102 | - |
dc.identifier.scopusid | 2-s2.0-85126882337 | - |
dc.identifier.wosid | 000691088900002 | - |
dc.identifier.bibliographicCitation | Journal of Biomedical Nanotechnology, v.17, no.7, pp.1273 - 1283 | - |
dc.relation.isPartOf | Journal of Biomedical Nanotechnology | - |
dc.citation.title | Journal of Biomedical Nanotechnology | - |
dc.citation.volume | 17 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1273 | - |
dc.citation.endPage | 1283 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | AMPHIPHILIC PEPTIDES | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | ARGININE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | SIRNA | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | MUCUS | - |
dc.subject.keywordPlus | HMGB1 | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | Acute Lung Injury | - |
dc.subject.keywordAuthor | RP1 | - |
dc.subject.keywordAuthor | Delivery | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | MicroRNA-92a | - |
dc.identifier.url | https://www.ingentaconnect.com/content/asp/jbn/2021/00000017/00000007/art00002;jsessionid=h8bestataeic.x-ic-live-03 | - |
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