Homologous sense and antisense expression of a gene in Dunaliella tertiolecta
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, Jing Yue | - |
dc.contributor.author | Jeon, Hancheol | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.contributor.author | Lee, Yew | - |
dc.contributor.author | Jin, EonSeon | - |
dc.date.accessioned | 2022-07-07T04:30:17Z | - |
dc.date.available | 2022-07-07T04:30:17Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 0032-0935 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143127 | - |
dc.description.abstract | Our aim was to investigate the potential of antisense expression and overexpression of a specific gene, the carotenoid biosynthesis-related (CBR) gene, in the microalgae Dunaliella. DNA amplified from sense and antisense vector constructs was used to transform Dunaliella tertiolecta. The Gateway vector for plant transformation was used to make an expression cassette, and the essential region for Dunaliella transformation was amplified by PCR and used for transformation. The transformation efficiency using a 3.2 kb PCR product was 130 transformants/mu g DNA for both sense and antisense transformations. Among 200 BASTA-resistant colonies from the sense transformation and antisense transformation, separately, nine positive transformants for sense expression and five positive transformants for the antisense expression were obtained by genomic DNA PCR. The insertion was also verified by genomic Southern analysis. Among five positive sense transformants, one transformant was tested and verified for the overexpression of CBR-GFP fusion protein by Western blot analysis. One of the five antisense transformants showed almost complete inhibition of gene expression under light stress conditions (400 mu mol photons m(-2) s(-1)) as determined by quantitative RT-PCR and Western blot analysis. Although there was no difference in the growth patterns or photosynthetic parameters between the wild type (including the vector control) and transformants, the zeaxanthin content of the antisense CBR mutant was lowered under light stress conditions. Thus, we show that the sense and antisense RNA technology can be easily and strategically used for the functional analysis of interesting gene in D. tertiolecta. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | Homologous sense and antisense expression of a gene in Dunaliella tertiolecta | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jin, EonSeon | - |
dc.identifier.doi | 10.1007/s00425-015-2369-2 | - |
dc.identifier.scopusid | 2-s2.0-84941174894 | - |
dc.identifier.wosid | 000360889600021 | - |
dc.identifier.bibliographicCitation | PLANTA, v.242, no.4, pp.1051 - 1058 | - |
dc.relation.isPartOf | PLANTA | - |
dc.citation.title | PLANTA | - |
dc.citation.volume | 242 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1051 | - |
dc.citation.endPage | 1058 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | SALINA | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | ALGAE | - |
dc.subject.keywordAuthor | Dunaliella transformation | - |
dc.subject.keywordAuthor | Antisense expression | - |
dc.subject.keywordAuthor | Carotenoid biosynthesis-related protein | - |
dc.subject.keywordAuthor | Overexpression | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s00425-015-2369-2 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.