An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses
DC Field | Value | Language |
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dc.contributor.author | Gwak, Yunho | - |
dc.contributor.author | Jung, Woongsic | - |
dc.contributor.author | Lee, Yew | - |
dc.contributor.author | Kim, Ji Sook | - |
dc.contributor.author | Kim, Chul Geun | - |
dc.contributor.author | Ju, Ji-Hyun | - |
dc.contributor.author | Song, Chihong | - |
dc.contributor.author | Hyun, Jae-Kyung | - |
dc.contributor.author | Jin, EonSeon | - |
dc.date.accessioned | 2022-07-07T07:44:21Z | - |
dc.date.available | 2022-07-07T07:44:21Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-11 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/143965 | - |
dc.description.abstract | The structure and function of the Antarctic marine diatom Chaetoceros neogracile antifreeze protein (Cn-AFP), as well as its expression levels and characteristics of the ice-binding site, were analyzed in the present study. In silico analysis revealed that the Cn-AFP promoter contains both light- and temperature-responsive elements. Northern and Western blot analyses demonstrated that both Cn-AFP transcript and protein expression were strongly and rapidly stimulated by freezing, as well as temperature and high light stress. Immunogold labeling revealed that Cn-AFP is preferentially localized to the intracellular space near the chloroplast membrane. Recombinant Cn-AFP had clear antifreeze activity. Protein-folding simulation was used to predict the putative ice-binding sites in Cn-AFP, and site-directed mutagenesis of the Cn-AFP b-face confirmed their identification.Gwak, Y., Jung, W., Lee, Y., Kim, J. S., Kim, C. G., Ju, J.-H., Song, C., Hyun, J.-K., Jin, E. An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.title | An intracellular antifreeze protein from an Antarctic microalga that responds to various environmental stresses | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chul Geun | - |
dc.contributor.affiliatedAuthor | Jin, EonSeon | - |
dc.identifier.doi | 10.1096/fj.14-256388 | - |
dc.identifier.scopusid | 2-s2.0-84912002069 | - |
dc.identifier.wosid | 000344050900029 | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, v.28, no.11, pp.4924 - 4935 | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.citation.title | FASEB JOURNAL | - |
dc.citation.volume | 28 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 4924 | - |
dc.citation.endPage | 4935 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | ICE-BINDING PROTEINS | - |
dc.subject.keywordPlus | CIS-ACTING ELEMENTS | - |
dc.subject.keywordPlus | SEA-ICE | - |
dc.subject.keywordPlus | REGULATORY ELEMENTS | - |
dc.subject.keywordPlus | STRUCTURE PREDICTION | - |
dc.subject.keywordPlus | DEHYDRATION STRESS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordAuthor | Chaetoceros neogracile | - |
dc.subject.keywordAuthor | immunogold labeling | - |
dc.subject.keywordAuthor | ice-binding site | - |
dc.identifier.url | https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.14-256388 | - |
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