Increased FOXM1 Expression by Cisplatin Inhibits Paclitaxel-Related Apoptosis in Cisplatin-Resistant Human Oral Squamous Cell Carcinoma (OSCC) Cell Lines
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hyeong Sim | - |
dc.contributor.author | Kim, Young-Kyun | - |
dc.contributor.author | Hwang, Kyung-Gyun | - |
dc.contributor.author | Yun, Pil-Young | - |
dc.date.accessioned | 2022-07-07T09:27:03Z | - |
dc.date.available | 2022-07-07T09:27:03Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/144221 | - |
dc.description.abstract | Cisplatin and paclitaxel are commonly used to treat oral cancer, but their use is often limited because of acquired drug resistance. Here, we tested the effects of combined cisplatin and paclitaxel on three parental (YD-8, YD-9, and YD-38) and three cisplatin-resistant (YD-8/CIS, YD-9/CIS, and YD-38/CIS) oral squamous cell carcinoma (OSCC) cell lines using cell proliferation assays and combination index analysis. We detected forkhead box protein M1 (FOXM1) mRNA and protein expression via real-time qPCR and Western blot assays. Cell death of the cisplatin-resistant cell lines in response to these drugs with or without a FOXM1 inhibitor (forkhead domain inhibitory compound 6) was then measured by propidium iodide staining and TdT dUTP nick end labeling (TUNEL) assays. In all six OSCC cell lines, cell growth was more inhibited by paclitaxel alone than combination therapy. Cisplatin-induced overexpression of FOXM1 showed the same trend only in cisplatin-resistant cell lines, indicating that it was associated with inhibition of paclitaxel-related apoptosis. In summary, these results suggest that, in three cisplatin-resistant cell lines, the combination of cisplatin and paclitaxel had an antagonistic effect, likely because cisplatin blocks paclitaxel-induced apoptosis. Cisplatin-induced FOXM1 overexpression may explain the failure of this combination. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Increased FOXM1 Expression by Cisplatin Inhibits Paclitaxel-Related Apoptosis in Cisplatin-Resistant Human Oral Squamous Cell Carcinoma (OSCC) Cell Lines | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Kyung-Gyun | - |
dc.identifier.doi | 10.3390/ijms21238897 | - |
dc.identifier.scopusid | 2-s2.0-85096595780 | - |
dc.identifier.wosid | 000597908700001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.23, pp.1 - 12 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | cisplatin | - |
dc.subject.keywordPlus | complementary DNA | - |
dc.subject.keywordPlus | forkhead box protein M1 | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase | - |
dc.subject.keywordPlus | paclitaxel | - |
dc.subject.keywordPlus | antineoplastic agent | - |
dc.subject.keywordPlus | cisplatin | - |
dc.subject.keywordPlus | forkhead box protein M1 | - |
dc.subject.keywordPlus | FOXM1 protein, human | - |
dc.subject.keywordPlus | paclitaxel | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | cancer combination chemotherapy | - |
dc.subject.keywordPlus | cancer resistance | - |
dc.subject.keywordPlus | cell death | - |
dc.subject.keywordPlus | cell proliferation | - |
dc.subject.keywordPlus | cell viability | - |
dc.subject.keywordPlus | cisplatin-resistant cell line | - |
dc.subject.keywordPlus | combination index | - |
dc.subject.keywordPlus | DNA strand breakage | - |
dc.subject.keywordPlus | down regulation | - |
dc.subject.keywordPlus | flow cytometry | - |
dc.subject.keywordPlus | fluorometry | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | mouth squamous cell carcinoma | - |
dc.subject.keywordPlus | oral squamous cell carcinoma cell line | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | real time polymerase chain reaction | - |
dc.subject.keywordPlus | RNA isolation | - |
dc.subject.keywordPlus | TUNEL assay | - |
dc.subject.keywordPlus | upregulation | - |
dc.subject.keywordPlus | Western blotting | - |
dc.subject.keywordPlus | yd 38 cisplatin-resistant cell line | - |
dc.subject.keywordPlus | yd 38 oral squamous cell carcinoma cell line | - |
dc.subject.keywordPlus | yd 8 cisplatin-resistant cell line | - |
dc.subject.keywordPlus | yd 8 oral squamous cell carcinoma cell line | - |
dc.subject.keywordPlus | yd 9 cisplatin-resistant cell line | - |
dc.subject.keywordPlus | yd 9 oral squamous cell carcinoma cell line | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | drug effect | - |
dc.subject.keywordPlus | drug resistance | - |
dc.subject.keywordPlus | drug therapy | - |
dc.subject.keywordAuthor | OSCC | - |
dc.subject.keywordAuthor | cisplatin | - |
dc.subject.keywordAuthor | paclitaxel | - |
dc.subject.keywordAuthor | FOXM1 | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/21/23/8897 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.