Bacteroides fragilisEnterotoxin Induces Sulfiredoxin-1 Expression in Intestinal Epithelial Cell Lines Through a Mitogen-Activated Protein Kinases- and Nrf2-Dependent Pathway, Leading to the Suppression of Apoptosis
DC Field | Value | Language |
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dc.contributor.author | Jeon, Jong Ik | - |
dc.contributor.author | Choi, Jun Ho | - |
dc.contributor.author | Lee, Keun Hwa | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.date.accessioned | 2022-07-07T17:29:06Z | - |
dc.date.available | 2022-07-07T17:29:06Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/145298 | - |
dc.description.abstract | EnterotoxigenicBacteroides fragilisis a causative agent of colitis and secrets enterotoxin (BFT), leading to the disease. Sulfiredoxin (Srx)-1 serves to protect from oxidative damages. Although BFT can generate reactive oxygen species in intestinal epithelial cells (IECs), no Srx-1 expression has been reported in ETBF infection. In this study, we explored the effects of ETBF-produced BFT on Srx-1 induction in IECs. Treatment of IECs with BFT resulted in increased expression of Srx-1 in a time-dependent manner. BFT treatment also activated transcriptional signals including Nrf2, AP-1 and NF-kappa B, and the Srx-1 induction was dependent on the activation of Nrf2 signals. Nrf2 activation was assessed using immunoblot and Nrf2-DNA binding activity and the specificity was confirmed by supershift and competition assays. Suppression of NF-kappa B or AP-1 signals did not affect the upregulation of Srx-1 expression. Nrf2-dependent Srx-1 expression was associated with the activation of p38 mitogen-activated protein kinases (MAPKs) in IECs. Furthermore, suppression of Srx-1 significantly enhanced apoptosis while overexpression of Srx-1 significantly attenuated apoptosis during exposure to BFT. These results imply that a signaling cascade involving p38 and Nrf2 is essential for Srx-1 upregulation in IECs stimulated with BFT. Following this upregulation, Srx-1 may control the apoptosis in BFT-exposed IECs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Bacteroides fragilisEnterotoxin Induces Sulfiredoxin-1 Expression in Intestinal Epithelial Cell Lines Through a Mitogen-Activated Protein Kinases- and Nrf2-Dependent Pathway, Leading to the Suppression of Apoptosis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Keun Hwa | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.identifier.doi | 10.3390/ijms21155383 | - |
dc.identifier.scopusid | 2-s2.0-85088938717 | - |
dc.identifier.wosid | 000559062600001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.15, pp.1 - 17 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 17 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | REGULATES HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | B-DEPENDENT PATHWAY | - |
dc.subject.keywordPlus | FRAGILIS ENTEROTOXIN | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | REDUCTASE | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordAuthor | Bacteroides fragilis | - |
dc.subject.keywordAuthor | enterotoxin | - |
dc.subject.keywordAuthor | intestinal epithelial cells | - |
dc.subject.keywordAuthor | sulfiredoxin-1 | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/21/15/5383 | - |
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