Hypoxia-Associated Changes in Striatal Tonic Dopamine Release: Real-Timein vivoMeasurements With a Novel Voltammetry Technique
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Barath, Abhijeet S. | - |
dc.contributor.author | Rusheen, Aaron E. | - |
dc.contributor.author | Rojas Cabrera, Juan M. | - |
dc.contributor.author | Price, J. Blair | - |
dc.contributor.author | Owen, Robert L. | - |
dc.contributor.author | Shin, Hojin | - |
dc.contributor.author | Jang, Dong Pyo | - |
dc.contributor.author | Blaha, Charles D. | - |
dc.contributor.author | Lee, Kendall H. | - |
dc.contributor.author | Oh, Yoonbae | - |
dc.date.accessioned | 2022-07-07T17:33:23Z | - |
dc.date.available | 2022-07-07T17:33:23Z | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1662-4548 | - |
dc.identifier.issn | 1662-453X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/145326 | - |
dc.description.abstract | Introduction Striatal tonic dopamine increases rapidly during global cerebral hypoxia. This phenomenon has previously been studied using microdialysis techniques which have relatively poor spatio-temporal resolution. In this study, we measured changes in tonic dopamine during hypoxia (death) in real time with high spatio-temporal resolution using novel multiple cyclic square wave voltammetry (MCSWV) and conventional fast scan cyclic voltammetry (FSCV) techniques. Methods MCSWV and FSCV were used to measure dopamine release at baseline and during hypoxia induced by euthanasia, with and without prior alpha-methyl-p-tyrosine (AMPT) treatment, in urethane anesthetized male Sprague-Dawley rats. Results Baseline tonic dopamine levels were found to be 274.1 +/- 49.4 nM (n= 5; mean +/- SEM). Following intracardiac urethane injection, the tonic levels increased to a peak concentration of 1753.8 +/- 95.7 nM within 3.6 +/- 0.6 min (n= 5), followed by a decline to 50.7 +/- 21.5 nM (n= 4) at 20 min. AMPT pre-treatment significantly reduced this dopamine peak to 677.9 +/- 185.7 nM (n= 3). FSCV showed a significantly higher (p= 0.0079) peak dopamine release of 6430.4 +/- 1805.7 nM (n= 5) during euthanasia-induced cerebral hypoxia. Conclusion MCSWV is a novel tool to study rapid changes in tonic dopamine releasein vivoduring hypoxia. We found a 6-fold increase in peak dopamine levels during hypoxia which was attenuated with AMPT pre-treatment. These changes are much lower compared to those found with microdialysis. This could be due to improved estimation of baseline tonic dopamine with MCSWV. Higher dopamine response measured with FSCV could be due to an increased oxidation current from electroactive interferents. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | FRONTIERS MEDIA SA | - |
dc.title | Hypoxia-Associated Changes in Striatal Tonic Dopamine Release: Real-Timein vivoMeasurements With a Novel Voltammetry Technique | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3389/fnins.2020.00869 | - |
dc.identifier.scopusid | 2-s2.0-85090039710 | - |
dc.identifier.wosid | 000568298700001 | - |
dc.identifier.bibliographicCitation | FRONTIERS IN NEUROSCIENCE, v.14, pp 1 - 8 | - |
dc.citation.title | FRONTIERS IN NEUROSCIENCE | - |
dc.citation.volume | 14 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | CAROTID-BODY | - |
dc.subject.keywordPlus | EXTRACELLULAR DOPAMINE | - |
dc.subject.keywordPlus | SUBSTANTIA-NIGRA | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | VULNERABILITY | - |
dc.subject.keywordPlus | TRANSMISSION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | ANOXIA | - |
dc.subject.keywordAuthor | hypoxia | - |
dc.subject.keywordAuthor | striatum | - |
dc.subject.keywordAuthor | rodent | - |
dc.subject.keywordAuthor | tonic dopamine | - |
dc.subject.keywordAuthor | voltammetry | - |
dc.identifier.url | https://www.frontiersin.org/articles/10.3389/fnins.2020.00869/full | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.