Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
DC Field | Value | Language |
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dc.contributor.author | Joo, Hyun-Woo | - |
dc.contributor.author | Song, Yi-Sun | - |
dc.contributor.author | Park, In-Hwa | - |
dc.contributor.author | Shen, Guang-Yin | - |
dc.contributor.author | Seong, Jin-Hee | - |
dc.contributor.author | Shin, Na-Kyoung | - |
dc.contributor.author | Lee, A-Hyeon | - |
dc.contributor.author | Kim, Hyuck | - |
dc.contributor.author | Kim, Kyung-Soo | - |
dc.date.accessioned | 2022-07-07T22:19:06Z | - |
dc.date.available | 2022-07-07T22:19:06Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 2291-2789 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/145456 | - |
dc.description.abstract | Background. We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of beta-oxidation-related gene expression. However, the mechanisms underlying this process remain unclear. This study aimed to determine whether the improvement of hepatic steatosis by G-CSF was associated with autophagy in a rat model of diabetes.Methods. Eight rats were fed a standard diet, and 16 rats were fed high-fat diet (HFD) for 5 weeks. All HFD-fed rats were then injected with streptozotocin (STZ). One week later, HFD rats injected with STZ were randomly treated with either G-CSF (200 mu g/kg/day; diabetes mellitus (DM)/G-CSF) or saline (DM/saline) for 5 consecutive days. Four weeks later, serum biochemical and histology analyses were conducted. The expression of autophagy-associated proteins was determined by Western blotting. The mRNA expression of beta-oxidation-related genes was determined by quantitative real-time polymerase chain reaction. HepG2 cells were cultured under high glucose (HG) conditions with G-CSF treatment, followed by Oil Red O staining for quantification of lipids.Results. Histological analysis showed lower lipid accumulation in the DM/G-CSF group than in the DM/saline-treated rats. Protein levels of LC3 and beclin-1 were higher, and those of p62 were lower in the DM/G-CSF rats than in the DM/saline-treated rats. The mRNA expression of beta-oxidation-related genes was higher in DM/G-CSF rats than in the DM/saline-treated rats. Quantification of lipid levels in HepG2 cells cultured with HG and G-CSF treatment revealed no significant differences.Conclusions. Our data suggested that G-CSF potentially improves hepatic steatosis and autophagy in the liver of diabetic rats. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HINDAWI LTD | - |
dc.title | Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyuck | - |
dc.identifier.doi | 10.1155/2020/2156829 | - |
dc.identifier.scopusid | 2-s2.0-85089306511 | - |
dc.identifier.wosid | 000559259600001 | - |
dc.identifier.bibliographicCitation | CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, v.2020, pp.1 - 9 | - |
dc.relation.isPartOf | CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.title | CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.volume | 2020 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | FATTY LIVER-DISEASE | - |
dc.subject.keywordPlus | METABOLIC SYNDROME | - |
dc.subject.keywordPlus | LIPOPHAGY | - |
dc.subject.keywordPlus | PREVENTS | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | RISK | - |
dc.identifier.url | https://www.hindawi.com/journals/cjgh/2020/2156829/ | - |
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