Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sugita, Yoshihiko | - |
dc.contributor.author | Okubo, Takahisa | - |
dc.contributor.author | Saita, Makiko | - |
dc.contributor.author | Ishijima, Manabu | - |
dc.contributor.author | Torii, Yasuyoshi | - |
dc.contributor.author | Tanaka, Miyuki | - |
dc.contributor.author | Iwasaki, Chika | - |
dc.contributor.author | Sekiya, Takeo | - |
dc.contributor.author | Tabuchi, Masako | - |
dc.contributor.author | Rezaei, Naser Mohammadzadeh | - |
dc.contributor.author | Taniyama, Takashi | - |
dc.contributor.author | Sato, Nobuaki | - |
dc.contributor.author | Saruta, Juri | - |
dc.contributor.author | Hasegawa, Masakazu | - |
dc.contributor.author | Hirota, Makoto | - |
dc.contributor.author | Park, Wonhee | - |
dc.contributor.author | Lee, Masaichi Chang-Il | - |
dc.contributor.author | Maeda, Hatsuhiko | - |
dc.contributor.author | Ogawa, Takahiro | - |
dc.date.accessioned | 2022-07-08T08:39:37Z | - |
dc.date.available | 2022-07-08T08:39:37Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/145954 | - |
dc.description.abstract | Poly(methyl methacrylate) (PMMA)-based bone cement, which is widely used to affix orthopedic metallic implants, is considered bio-tolerant but lacks osteoconductivity and is cytotoxic. Implant loosening and toxic complications are significant and recognized problems. Here we devised two strategies to improve PMMA-based bone cement: (1) adding 4-methacryloyloxylethyl trimellitate anhydride (4-META) to MMA monomer to render it hydrophilic; and (2) using tri-n-butyl borane (TBB) as a polymerization initiator instead of benzoyl peroxide (BPO) to reduce free radical production. Rat bone marrow-derived osteoblasts were cultured on PMMA-BPO, common bone cement ingredients, and 4-META/MMA-TBB, newly formulated ingredients. After 24 h of incubation, more cells survived on 4-META/MMA-TBB than on PMMA-BPO. The mineralized area was 20-times greater on 4-META/MMA-TBB than PMMA-BPO at the later culture stage and was accompanied by upregulated osteogenic gene expression. The strength of bone-to-cement integration in rat femurs was 4- and 7-times greater for 4-META/MMA-TBB than PMMA-BPO during early- and late-stage healing, respectively. MicroCT and histomorphometric analyses revealed contact osteogenesis exclusively around 4-META/MMA-TBB, with minimal soft tissue interposition. Hydrophilicity of 4-META/MMA-TBB was sustained for 24 h, particularly under wet conditions, whereas PMMA-BPO was hydrophobic immediately after mixing and was unaffected by time or condition. Electron spin resonance (ESR) spectroscopy revealed that the free radical production for 4-META/MMA-TBB was 1/10 to 1/20 that of PMMA-BPO within 24 h, and the substantial difference persisted for at least 10 days. The compromised ability of PMMA-BPO in recruiting cells was substantially alleviated by adding free radical-scavenging amino-acid N-acetyl cysteine (NAC) into the material, whereas adding NAC did not affect the ability of 4-META/MMA-TBB. These results suggest that 4-META/MMA-TBB shows significantly reduced cytotoxicity compared to PMMA-BPO and induces osteoconductivity due to uniquely created hydrophilic and radical-free interface. Further pre-clinical and clinical validations are warranted. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Novel Osteogenic Behaviors around Hydrophilic and Radical-Free 4-META/MMA-TBB: Implications of an Osseointegrating Bone Cement | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Wonhee | - |
dc.identifier.doi | 10.3390/ijms21072405 | - |
dc.identifier.scopusid | 2-s2.0-85083042241 | - |
dc.identifier.wosid | 000535574200147 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.7, pp.1 - 24 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 24 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | N-ACETYL CYSTEINE | - |
dc.subject.keywordPlus | MECHANICAL-PROPERTIES | - |
dc.subject.keywordPlus | FEMORAL-NECK | - |
dc.subject.keywordPlus | UV-PHOTOFUNCTIONALIZATION | - |
dc.subject.keywordPlus | INTERNAL-FIXATION | - |
dc.subject.keywordPlus | RESIN | - |
dc.subject.keywordPlus | TITANIUM | - |
dc.subject.keywordPlus | POLYMETHYLMETHACRYLATE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | arthroplasty | - |
dc.subject.keywordAuthor | total hip replacement | - |
dc.subject.keywordAuthor | free radical | - |
dc.subject.keywordAuthor | PMMA | - |
dc.subject.keywordAuthor | cytotoxicity | - |
dc.subject.keywordAuthor | implants | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/21/7/2405 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.