Optimization of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells using response surface methodology
DC Field | Value | Language |
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dc.contributor.author | Kwon, Soon-Sun | - |
dc.contributor.author | Kim, Hyang | - |
dc.contributor.author | Shin, Sang-Jin | - |
dc.contributor.author | Lee, Seung Yeol | - |
dc.date.accessioned | 2022-07-08T12:35:15Z | - |
dc.date.available | 2022-07-08T12:35:15Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-03 | - |
dc.identifier.issn | 1749-799X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/146105 | - |
dc.description.abstract | Background In order to optimize the tenogenic differentiation of mesenchymal stem cells (MSCs), researchers should consider various factors. However, this requires testing numerous experimental settings, which is costly and time-consuming. We aimed to assess the differential effects of transforming growth factor beta-3 (TGF-β3) on the tenogenesis of tonsil-derived MSCs (T-MSCs) and bone marrow-derived MSCs (BM-MSCs) using response surface methodology (RSM). Methods Bone marrow and tonsillar tissue were collected from four patients; mononuclear cells were separated and treated with 5 or 10 ng/mL of TGF-β3. A full factorial experimental design with a categorical factor of 0 was employed to study the effect of tension based on T-MSCs. Eighty-four trials were fitted with RSM and then used to obtain mathematical prediction models. Results Exposure of T-MSCs and BM-MSCs to TGF-β3 increased the expression of scleraxis (SCX), tenomodulin (TNMD), decorin, collagen I, and tenascin C. Expression of most of these factors reached a maximum after 2–3 days of treatment. The model predicted that the values of the tenocyte lineage-related factors assessed would be significantly increased at 2.5 days of culture with 2.7 ng/mL of TGF-β3 for T-MSCs and at 2.3 days of culture regardless of TGF-β3 concentration for BM-MSCs. Conclusions This study demonstrated that the RSM prediction of the culture time necessary for the tenogenic differentiation of T-MSCs and BM-MSCs under TGF-β3 stimulation was similar to the experimentally determined time of peak expression of tenocyte-related mRNAs, suggesting the potential of using the RSM approach for optimization of the culture protocol for tenogenesis of MSCs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.title | Optimization of tenocyte lineage-related factors from tonsil-derived mesenchymal stem cells using response surface methodology | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Seung Yeol | - |
dc.identifier.doi | 10.1186/s13018-020-01623-8 | - |
dc.identifier.scopusid | 2-s2.0-85082019281 | - |
dc.identifier.wosid | 000522033400001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH, v.15, no.1, pp.1 - 9 | - |
dc.relation.isPartOf | JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH | - |
dc.citation.title | JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH | - |
dc.citation.volume | 15 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Orthopedics | - |
dc.relation.journalWebOfScienceCategory | Orthopedics | - |
dc.subject.keywordPlus | TENOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | GROWTH-FACTORS | - |
dc.subject.keywordPlus | TENDON INJURY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | CCN1 | - |
dc.subject.keywordAuthor | Tenocyte | - |
dc.subject.keywordAuthor | Tonsil-derived mesenchymal stem cells | - |
dc.subject.keywordAuthor | Bone marrow-derived mesenchymal stem cells | - |
dc.subject.keywordAuthor | Design of experiments | - |
dc.subject.keywordAuthor | Response surface methodology | - |
dc.identifier.url | https://josr-online.biomedcentral.com/articles/10.1186/s13018-020-01623-8 | - |
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