Bcl-2 Overexpression Induces Neurite Outgrowth via the Bmp4/Tbx3/NeuroD1 Cascade in H19-7 Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Yun Young | - |
dc.contributor.author | Choi, Hye-jin | - |
dc.contributor.author | Lee, So Young | - |
dc.contributor.author | Park, Shin-Young | - |
dc.contributor.author | Kang, Min-Jeong | - |
dc.contributor.author | Han, Jinil | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.date.accessioned | 2022-07-08T16:04:01Z | - |
dc.date.available | 2022-07-08T16:04:01Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 0272-4340 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/146344 | - |
dc.description.abstract | Bcl-2 is overexpressed in the nervous system during neural development and plays an important role in modulating cell survival. In addition to its anti-apoptotic function, it has been suggested previously that Bcl-2 might act as a mediator of neuronal differentiation. However, the mechanism by which Bcl-2 might influence neurogenesis is not sufficiently understood. In this study, we aimed to determine the non-apoptotic functions of Bcl-2 during neuronal differentiation. First, we used microarrays to analyze the whole-genome expression patterns of rat neural stem cells overexpressing Bcl-2 and found that Bcl-2 overexpression induced the expression of various neurogenic genes. Moreover, Bcl-2 overexpression increased the neurite length as well as expression of Bmp4, Tbx3, and proneural basic helix-loop-helix genes, such as NeuroD1, NeuroD2, and Mash1, in H19-7 rat hippocampal precursor cells. To determine the hierarchy of these molecules, we selectively depleted Bmp4, Tbx3, and NeuroD1 in Bcl-2-overexpressing cells. Bmp4 depletion suppressed the upregulation of Tbx3 and NeuroD1 as well as neurite outgrowth, which was induced by Bcl-2 overexpression. Although Tbx3 knockdown repressed Bcl-2-mediated neurite elaboration and downregulated NeuroD1 expression, it did not affect Bcl-2-induced Bmp4 expression. While the depletion of NeuroD1 had no effect on the expression of Bcl-2, Bmp4, or Tbx3, Bcl-2-mediated neurite outgrowth was suppressed. Taken together, these results demonstrate that Bcl-2 regulates neurite outgrowth through the Bmp4/Tbx3/NeuroD1 cascade in H19-7 cells, indicating that Bcl-2 may have a direct role in neuronal development in addition to its well-known anti-apoptotic function in response to environmental insults. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER/PLENUM PUBLISHERS | - |
dc.title | Bcl-2 Overexpression Induces Neurite Outgrowth via the Bmp4/Tbx3/NeuroD1 Cascade in H19-7 Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Joong-Soo | - |
dc.identifier.doi | 10.1007/s10571-019-00732-1 | - |
dc.identifier.scopusid | 2-s2.0-85071849438 | - |
dc.identifier.wosid | 000511762600011 | - |
dc.identifier.bibliographicCitation | CELLULAR AND MOLECULAR NEUROBIOLOGY, v.40, no.1, pp.153 - 166 | - |
dc.relation.isPartOf | CELLULAR AND MOLECULAR NEUROBIOLOGY | - |
dc.citation.title | CELLULAR AND MOLECULAR NEUROBIOLOGY | - |
dc.citation.volume | 40 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 153 | - |
dc.citation.endPage | 166 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | BONE MORPHOGENETIC PROTEINS | - |
dc.subject.keywordPlus | T-BOX GENES | - |
dc.subject.keywordPlus | NEURONAL DIFFERENTIATION | - |
dc.subject.keywordPlus | DOWNSTREAM TARGET | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | NEUROGENESIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PROTECTS | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordAuthor | Bcl-2 | - |
dc.subject.keywordAuthor | Neurite outgrowth | - |
dc.subject.keywordAuthor | Bmp4 | - |
dc.subject.keywordAuthor | Tbx3 | - |
dc.subject.keywordAuthor | NeuroD1 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs10571-019-00732-1 | - |
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