Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha, My Kieu | - |
dc.contributor.author | Chung, Kyung Hwun | - |
dc.contributor.author | Yoon, Tae Hyun | - |
dc.date.accessioned | 2022-07-08T16:04:27Z | - |
dc.date.available | 2022-07-08T16:04:27Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 2079-4991 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/146351 | - |
dc.description.abstract | Cellular association of nanoparticles (NPs) and their resultant cytotoxicity are heterogeneous in nature and can be influenced by the variances in NPs' properties, cell types, and status. However, conventional in vitro assays typically consider the administered NP dose and the averaged cellular responses based on the assumption of a uniform distribution of monodisperse NPs in homogeneous cells, which might be insufficient to describe the complex nature of cell-NP interactions. Here, using flow cytometry, we report observations of the heterogeneity in the cellular association of silver nanoparticles (AgNPs) in A549 cells, which resulted in distinct dose-response relationships and cytotoxicity. Type I and Type II cells were moderately associated with AgNPs but as the cellular AgNP dose increased, Type I cells remained viable while Type II cells became less viable. Type III cells did not have high affinity with AgNPs but were, however, the least viable. Transmission electron microscopic images revealed that the biodistribution and the released Ag+ ions contributed to the distinct toxic effects of AgNPs in different populations. This single-cell dose-response analysis approach enabled the examination of how differently individual cells responded to different cellular NP doses and provided insights into nanotoxicity pathways at a single-cell level. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Heterogeneity in Biodistribution and Cytotoxicity of Silver Nanoparticles in Pulmonary Adenocarcinoma Human Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoon, Tae Hyun | - |
dc.identifier.doi | 10.3390/nano10010036 | - |
dc.identifier.scopusid | 2-s2.0-85077364456 | - |
dc.identifier.wosid | 000516825600036 | - |
dc.identifier.bibliographicCitation | NANOMATERIALS, v.10, no.1, pp.1 - 13 | - |
dc.relation.isPartOf | NANOMATERIALS | - |
dc.citation.title | NANOMATERIALS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 13 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | CELLULAR UPTAKE | - |
dc.subject.keywordPlus | LIGHT SCATTER | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | NANOMATERIALS | - |
dc.subject.keywordPlus | GENOTOXICITY | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordPlus | LEVEL | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordAuthor | single-cell | - |
dc.subject.keywordAuthor | toxicity | - |
dc.subject.keywordAuthor | flow cytometry | - |
dc.subject.keywordAuthor | silver nanoparticles | - |
dc.identifier.url | https://www.mdpi.com/2079-4991/10/1/36 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.