Comparison of Direct Syringe Filtration and Membrane Filtration for the Selective Isolation of Campylobacter jejuni from Ready-to-Eat Sprouts
DC Field | Value | Language |
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dc.contributor.author | Chon, Jung-Whan | - |
dc.contributor.author | Kim, Dong-Hyeon | - |
dc.contributor.author | Bae, Dongryeoul | - |
dc.contributor.author | Song, Kwang-Young | - |
dc.contributor.author | Kim, Hyunsook | - |
dc.contributor.author | Sung, Kidon | - |
dc.contributor.author | Seo, Kun-Ho | - |
dc.date.accessioned | 2022-07-09T14:51:43Z | - |
dc.date.available | 2022-07-09T14:51:43Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 1535-3141 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/147744 | - |
dc.description.abstract | Culture method using enrichment broth and selective agar is one of the most common isolation methods for detecting Campylobacter jejuni from food. However, the overgrowth of competing bacteria in enrichment culture complicates the selective isolation of C. jejuni. In this study, we compared an enrichment/plating method for the isolation of C. jejuni from sprout samples with an enrichment/plating method with syringe or membrane filtration when transferring enriched broths to plates. Four types of sprout samples were artificially contaminated with various levels of C. jejuni and incubated in 100 mL of Bolton broth for 48 h. Enrichment broths were either directly transferred onto modified charcoal-cefoperazone-deoxycholate agar or filtered through membrane or with a syringe. A significantly higher (p < 0.05) isolation rate of Campylobacter positives was obtained with both filtration methods (58-61%) than with the method without filtration (10%). Membrane filtrations yielded 61%, whereas syringe yielded 58% positives. In most cases of unfiltered samples (98%), high competing flora covered most of the plate, making differentiation and picking of suspicious colonies difficult. However, less plates were contaminated with competing flora in both filtration methods. Only 5% of plates were contaminated in the syringe filtration method, whereas no competing flora was observed in membrane filtration (0%). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | Comparison of Direct Syringe Filtration and Membrane Filtration for the Selective Isolation of Campylobacter jejuni from Ready-to-Eat Sprouts | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunsook | - |
dc.identifier.doi | 10.1089/fpd.2018.2546 | - |
dc.identifier.scopusid | 2-s2.0-85066862318 | - |
dc.identifier.wosid | 000470693600001 | - |
dc.identifier.bibliographicCitation | FOODBORNE PATHOGENS AND DISEASE, v.16, no.6, pp.371 - 375 | - |
dc.relation.isPartOf | FOODBORNE PATHOGENS AND DISEASE | - |
dc.citation.title | FOODBORNE PATHOGENS AND DISEASE | - |
dc.citation.volume | 16 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 371 | - |
dc.citation.endPage | 375 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | PCR ASSAY | - |
dc.subject.keywordPlus | SALAD VEGETABLES | - |
dc.subject.keywordPlus | CULTURE METHOD | - |
dc.subject.keywordPlus | TIME PCR | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | SPP. | - |
dc.subject.keywordPlus | COLI | - |
dc.subject.keywordPlus | CONTAMINATION | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | EFFICACY | - |
dc.subject.keywordAuthor | Campylobacter | - |
dc.subject.keywordAuthor | syringe filter | - |
dc.subject.keywordAuthor | membrane filter | - |
dc.subject.keywordAuthor | sprout | - |
dc.subject.keywordAuthor | competing flora | - |
dc.identifier.url | https://www.liebertpub.com/doi/10.1089/fpd.2018.2546 | - |
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