Enhanced delivery of protein fused to cell penetrating peptides to mammalian cells
DC Field | Value | Language |
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dc.contributor.author | Moon, Jung-Il | - |
dc.contributor.author | Han, Min-Joon | - |
dc.contributor.author | Yu, Shin-Hye | - |
dc.contributor.author | Lee, Eun-Hye | - |
dc.contributor.author | Kim, Sang-Mi | - |
dc.contributor.author | Han, Kyuboem | - |
dc.contributor.author | Park, Chang-Hwan | - |
dc.contributor.author | Kim, Chun-Hyung | - |
dc.date.accessioned | 2022-07-09T18:06:19Z | - |
dc.date.available | 2022-07-09T18:06:19Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-05 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/147913 | - |
dc.description.abstract | Recent progress in cellular reprogramming technology and lineage-specific cell differentiation has provided great opportunities for translational research. Because virus-based gene delivery is not a practical reprogramming protocol, protein-based reprogramming has been receiving attention as a safe way to generate reprogrammed cells. However, the poor efficiency of the cellular uptake of reprogramming proteins is still a major obstacle. Here, we reported key factors which improve the cellular uptake of these proteins. Purified red fluorescent proteins fused with 9xLysine (dsRED-9K) as a cell penetrating peptide were efficiently delivered into the diverse primary cells. Protein delivery was improved by the addition of amodiaquine. Furthermore, purified dsRED-9K was able to penetrate all cell lineages derived from mouse embryonic stem cells efficiently. Our data may provide important insights into the design of protein-based reprogramming or differentiation protocols. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.title | Enhanced delivery of protein fused to cell penetrating peptides to mammalian cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Chang-Hwan | - |
dc.identifier.doi | 10.5483/BMBRep.2019.52.5.195 | - |
dc.identifier.scopusid | 2-s2.0-85066494535 | - |
dc.identifier.wosid | 000473211100005 | - |
dc.identifier.bibliographicCitation | BMB REPORTS, v.52, no.5, pp.324 - 329 | - |
dc.relation.isPartOf | BMB REPORTS | - |
dc.citation.title | BMB REPORTS | - |
dc.citation.volume | 52 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 324 | - |
dc.citation.endPage | 329 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002467510 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | TAT PROTEIN | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordAuthor | Amodiaquine (AQ) | - |
dc.subject.keywordAuthor | Cell Penetrating Peptide (CPP) | - |
dc.subject.keywordAuthor | Differentiation | - |
dc.subject.keywordAuthor | Polylysine (9K) | - |
dc.subject.keywordAuthor | Reprogramming | - |
dc.identifier.url | https://www.bmbreports.org/journal/view.html?doi=10.5483/BMBRep.2019.52.5.195 | - |
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