Supplementation of Modified Mannitol-Yolk-Polymyxin B Agar with Cefuroxime for Quantitative Detection of Bacillus cereus in Food

Abstract

The presence of unwanted competing flora has been the most common confounding factor in the enumeration of Bacillus cereus (B. cereus) using selective media such as mannitol-yolk-polymyxin B agar (MYPA). The objective of this study was to improve MYPA selectivity for B. cereus by supplementation with a second-generation cephalosporin, cefuroxime. The performance of cefuroxime-supplemented MYPA (cefu-MYPA) was evaluated by comparison with original MYPA in 60 food products with established microbiological standards for B. cereus contamination. Cefu-MYPA demonstrated superior recoverability and selectivity for B. cereus compared with original MYPA in most tested foods. B. cereus numbers on MYPA and cefu-MYPA were 363.5 and 462.0 CFU/g, respectively. Competing flora on cefu-MYPA was detected in significantly less samples (70%) compared to original MYPA (93%). In addition, the detection and isolation of suspected colonies were significantly improved in cefu-MYPA because of the reduction or elimination of competing flora in all tested foods except fruit juice, indicating superior selectivity of the modified medium. Our findings suggest that cefuroxime supplementation of MYPA would markedly improve the detection rate of B. cereus, particularly in foods with high levels of indigenous flora.