Reduced sirtuin 1/adenosine monophosphate-activated protein kinase in amyotrophic lateral sclerosis patient-derived mesenchymal stem cells can be restored by resveratrol
DC Field | Value | Language |
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dc.contributor.author | Yun, Young Chan | - |
dc.contributor.author | Jeong, Sin-Gu | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.contributor.author | Cho, Goang-Won | - |
dc.date.accessioned | 2022-07-10T14:54:55Z | - |
dc.date.available | 2022-07-10T14:54:55Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 1932-6254 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/148496 | - |
dc.description.abstract | Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease affecting motor neuron system. Our previous study has shown that bone marrow-mesenchymal stem cells (BM-MSCs) from ALS patients have functional limitations in releasing neurotrophic factors and exhibit the senescence phenotype. In this study, we examined sirtuin 1/adenosine monophosphate-activated protein kinase (SIRT1/AMPK) activities and identified significant decreases in the ALS-MSCs compared with normal healthy control originated BM-MSCs. This decline was restored by pretreatment with resveratrol (RSV), measured using quantitative polymerase chain reaction, NAD/NADH assay, and immunoblot analysis. Neuroprogenitor markers were increased in RSV-treated ALS-MSCs (RSV/ALS-MSCs). The differentiated ALS-MSCs (ALS-dMSCs) exhibited a cell body and dendritic shape similar to neurons. RSV/ALS-MSCs showed significantly increased differentiation rate as compared with the untreated ALS-dMSCs. The neurite numbers and lengths were also significantly increased. This was confirmed with immunoblot analysis using neuron specific markers such as nestin, NF-M, Tuj-1, and Map-2 in RSV/ALS-dMSCs. Thus, this study shows that ALS-MSCs showed down-regulation of AMPK/SIRT1 signalling, which was recovered by treatment with RSV. This data suggest that RSV can be one of the candidate agents for improving therapeutic efficacy of ALS patients' originated MSCs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Reduced sirtuin 1/adenosine monophosphate-activated protein kinase in amyotrophic lateral sclerosis patient-derived mesenchymal stem cells can be restored by resveratrol | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Hyun | - |
dc.identifier.doi | 10.1002/term.2776 | - |
dc.identifier.scopusid | 2-s2.0-85058167955 | - |
dc.identifier.wosid | 000456210800010 | - |
dc.identifier.bibliographicCitation | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.13, no.1, pp.110 - 115 | - |
dc.relation.isPartOf | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.title | JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 110 | - |
dc.citation.endPage | 115 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.subject.keywordPlus | PROMOTES NEURONAL DIFFERENTIATION | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | MARROW | - |
dc.subject.keywordPlus | SENESCENCE | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | AMPK | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordAuthor | amyotrophic lateral sclerosis | - |
dc.subject.keywordAuthor | mesenchymal stem cells | - |
dc.subject.keywordAuthor | neuronal differentiation | - |
dc.subject.keywordAuthor | resveratrol | - |
dc.subject.keywordAuthor | SIRT1 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/term.2776 | - |
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