The effect of cell penetrating peptide-conjugated coactivator-associated arginine methyltransferase 1 (CPP-CARM1) on the cloned mouse embryonic development
DC Field | Value | Language |
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dc.contributor.author | Bang, Jae-Il | - |
dc.contributor.author | Lee, Eun-Hye | - |
dc.contributor.author | Lee, Ah Reum | - |
dc.contributor.author | Lee, Jin Il | - |
dc.contributor.author | Choi, Seo Hye | - |
dc.contributor.author | Seol, Dong-Won | - |
dc.contributor.author | Park, Chang-Hwan | - |
dc.contributor.author | Lee, Dong Ryul | - |
dc.date.accessioned | 2022-07-11T00:38:00Z | - |
dc.date.available | 2022-07-11T00:38:00Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-11 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/149079 | - |
dc.description.abstract | Abnormalities in gene expression that negatively affect embryonic development are frequently observed in cloned embryos generated by somatic cell nuclear transfer (SCNT). In the present study, we successfully produced a cell-penetrating peptide (CPP)-conjugated with coactivator-associated arginine methyltransferase 1 (CARM1) protein from mammalian cells and confirmed introduction into donor somatic cells and cloned 8-cell embryos within 3 hours after addition to culture medium. In addition, H3R17 dimethylation and embryonic development up to the blastocyst stage were increased in the group treated with exogenous CPP-CARM1 protein compared with the untreated group (control). Interestingly, the number of total cells and trophectoderm in blastocysts as well as implantation rate were significantly increased in the CPP-CARM1 protein-treated group. However, the cell number of inner cell mass (ICM) was not changed compared with the control group; similarly, expression of pluripotency-related genes Oct4 and Nanog (ICM markers) was not significantly different between groups. On the other hand, expression of the implantation-related gene Cdx2 (trophectoderm marker) was transiently increased after treatment with CPP-CARM1 protein. On the basis of these results, we conclude that supplementation with exogenous CPP-CARM1 protein improves embryonic development of cloned embryos through regulation of histone methylation and gene expression. In addition, our results suggest that CPP-CARM1 protein may be a useful tool for strengthening implantation of mammalian embryos. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE RESEARCH | - |
dc.title | The effect of cell penetrating peptide-conjugated coactivator-associated arginine methyltransferase 1 (CPP-CARM1) on the cloned mouse embryonic development | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Chang-Hwan | - |
dc.identifier.doi | 10.1038/s41598-018-35077-0 | - |
dc.identifier.scopusid | 2-s2.0-85056448006 | - |
dc.identifier.wosid | 000449944500009 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.8, no.1, pp.1 - 9 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 8 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NUCLEAR TRANSFER | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | CLONING EFFICIENCY | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | METHYLATION | - |
dc.subject.keywordPlus | IMPROVES | - |
dc.subject.keywordPlus | ADULT | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | MICE | - |
dc.identifier.url | https://www.nature.com/articles/s41598-018-35077-0 | - |
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