Interleukin-1 beta promotes neuronal differentiation through the Wnt5a/RhoA/ JNK pathway in cortical neural precursor cells
DC Field | Value | Language |
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dc.contributor.author | Park, Shin-Young | - |
dc.contributor.author | Kang, Min-Jeong | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.date.accessioned | 2022-07-11T15:52:01Z | - |
dc.date.available | 2022-07-11T15:52:01Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-07 | - |
dc.identifier.issn | 1756-6606 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/149744 | - |
dc.description.abstract | Pro-inflammatory cytokine interleukin-1 beta (IL-1 beta) is a key mediator of inflammation and stress in the central nervous system (CNS), and is highly expressed in the developing brain. In this study, we investigated the possible role of IL-1 beta in neuronal differentiation of cortical neural precursor cells (NPCs). We showed that stimulation with IL-1 beta increased expression levels of neurotrophin-3 (NT3) and neurogenin 1 (Ngn1) and promoted neurite outgrowth. We also found that IL-1 beta increased mRNA and protein levels of Wnt5a. Knockdown of Wnt5a by transfection with Wnt5a siRNA inhibited IL-1 beta-induced neuronal differentiation. Moreover, IL-1 beta-induced Wnt5a expression was regulated by nuclear factor kappa B (NT-kappa B) activation, which is involved in IL-1 beta-mediated neuronal differentiation. To examine the role of Wnt5a in neuronal differentiation of NPCs, we exogenously added Wnt5a. We found that exogenous Wnt5a promotes neuronal differentiation, and activates the RhoA/Rho-associated kinase (ROCK)/c-jun N-terminai kinase (JNK) pathway. In addition, Wnt5a-induced neuronal differentiation was blocked by RhoA siRNA, as well as by a specific Rho-kinase inhibitor (Y27632) or a SAPK/JNK inhibitor (SP600125). Furthermore, treatment with RhoA siRNA, Y27632, or SP600125 suppressed the IL-1 beta-induced neuronal differentiation. Therefore, these results suggest that the sequential Wnt5a/RhoA/ROCK/JNK pathway is involved in IL-1 beta-induced neuronal differentiation of NPCs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.title | Interleukin-1 beta promotes neuronal differentiation through the Wnt5a/RhoA/ JNK pathway in cortical neural precursor cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Joong-Soo | - |
dc.identifier.doi | 10.1186/s13041-018-0383-6 | - |
dc.identifier.scopusid | 2-s2.0-85049499348 | - |
dc.identifier.wosid | 000437501600001 | - |
dc.identifier.bibliographicCitation | MOLECULAR BRAIN, v.11, pp.1 - 12 | - |
dc.relation.isPartOf | MOLECULAR BRAIN | - |
dc.citation.title | MOLECULAR BRAIN | - |
dc.citation.volume | 11 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | FOCAL CEREBRAL-ISCHEMIA | - |
dc.subject.keywordPlus | NERVE GROWTH-FACTOR | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | ARTICULAR CHONDROCYTES | - |
dc.subject.keywordPlus | HIPPOCAMPAL-NEURONS | - |
dc.subject.keywordPlus | NEURITE OUTGROWTH | - |
dc.subject.keywordPlus | SPINAL-CORD | - |
dc.subject.keywordPlus | PC12 CELLS | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordAuthor | Interleukin-1 beta (IL-1β) | - |
dc.subject.keywordAuthor | Neuronal differentiation | - |
dc.subject.keywordAuthor | Wnt5a | - |
dc.subject.keywordAuthor | RhoA | - |
dc.subject.keywordAuthor | C-jun N-terminal kinase (JNK) | - |
dc.identifier.url | https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-018-0383-6 | - |
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