Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Yong Seung | - |
dc.contributor.author | Jung, Woon Yong | - |
dc.contributor.author | Heo, Hyejung | - |
dc.contributor.author | Park, Min Geun | - |
dc.contributor.author | Oh, Seung-Hun | - |
dc.contributor.author | Park, Byong-Gon | - |
dc.contributor.author | Kim, Soonhag | - |
dc.date.accessioned | 2022-07-12T07:34:07Z | - |
dc.date.available | 2022-07-12T07:34:07Z | - |
dc.date.created | 2021-05-14 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/150354 | - |
dc.description.abstract | Exosomes, naturally secreted nanoparticles, have been introduced as vehicles for horizontal transfer of genetic material. We induced autologous exosomes containing a cocktail of reprogramming factors ("reprosomes") to convert fibroblasts into neural progenitor cells (NPCs). The fibroblasts were treated with ultrasound and subsequently cultured in neural stem cell medium for 1 day to induce the release of reprosomes composed of reprogramming factors associated with chromatin remodeling and neural lineage-specific factors. After being treated with reprosomes, fibroblasts were converted into NPCs (rNPCs) with great efficiency via activation of chromatin remodeling, so quickly that only 5 days were required for the formation of 1500 spheroids showing an NPC-like phenotype. The rNPCs maintained self-renewal and proliferative properties for several weeks and successfully differentiated into neurons, astrocytes, and oligodendrocytes in vitro and in vivo. Reprosome-mediated cellular reprogramming is simple, safe, and efficient to produce autologous stem cells for clinical application. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Exosome-Mediated Ultra-Effective Direct Conversion of Human Fibroblasts into Neural Progenitor-like Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jung, Woon Yong | - |
dc.identifier.doi | 10.1021/acsnano.7b08297 | - |
dc.identifier.scopusid | 2-s2.0-85044535276 | - |
dc.identifier.wosid | 000428972600046 | - |
dc.identifier.bibliographicCitation | ACS NANO, v.12, no.3, pp.2531 - 2538 | - |
dc.relation.isPartOf | ACS NANO | - |
dc.citation.title | ACS NANO | - |
dc.citation.volume | 12 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 2531 | - |
dc.citation.endPage | 2538 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | HORIZONTAL TRANSFER | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | MICROVESICLES | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | ultrasound | - |
dc.subject.keywordAuthor | exosome | - |
dc.subject.keywordAuthor | fibroblast | - |
dc.subject.keywordAuthor | cellular reprogramming | - |
dc.subject.keywordAuthor | chromatin remodeling | - |
dc.subject.keywordAuthor | neural progenitor | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsnano.7b08297 | - |
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