Lin28B and miR-142-3p regulate neuronal differentiation by modulating Staufen1 expressionopen access
- Authors
- Oh, Younseo; Park, Jungyun; Kim, Jin-Il; Chang, Mi-Yoon; Lee, Sang-Hun; Cho, Youl-Hee; Hwang, Jungwook
- Issue Date
- Feb-2018
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- CELL DEATH AND DIFFERENTIATION, v.25, no.2, pp.432 - 443
- Indexed
- SCIE
SCOPUS
- Journal Title
- CELL DEATH AND DIFFERENTIATION
- Volume
- 25
- Number
- 2
- Start Page
- 432
- End Page
- 443
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/150587
- DOI
- 10.1038/cdd.2017.182
- ISSN
- 1350-9047
- Abstract
- Staufen1 (STAU1) and Lin28B are RNA-binding proteins that are involved in neuronal differentiation as a function of post-transcriptional regulation. STAU1 triggers post-transcriptional regulation, including mRNA export, mRNA relocation, translation and mRNA decay. Lin28B also has multiple functions in miRNA biogenesis and the regulation of translation. Here, we examined the connection between STAU1 and Lin28B and found that Lin28B regulates the abundance of STAU1 mRNA via miRNA maturation. Decreases in the expression of both STAU1 and Lin28B were observed during neuronal differentiation. Depletion of STAU1 or Lin28B inhibited neuronal differentiation, and overexpression of STAU1 or Lin28B enhanced neuronal differentiation. Interestingly, the stability of STAU1 mRNA was modulated by miR-142-3p, whose maturation was regulated by Lin28B. Thus, miR-142-3p expression increased as Lin28B expression decreased during differentiation, leading to the reduction of STAU1 expression. The transcriptome from Staufen-mediated mRNA decay (SMD) targets during differentiation was analyzed, confirming that STAU1 was a key factor in neuronal differentiation. In support of this finding, regulation of STAU1 expression in mouse neural precursor cells had the same effects on neuronal differentiation as it did in human neuroblastoma cells. These results revealed the collaboration of two RNA-binding proteins, STAU1 and Lin28B, as a regulatory mechanism in neuronal differentiation.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의생명공학전문대학원 > 서울 의생명과학과 > 1. Journal Articles
- 서울 의과대학 > 서울 유전학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/150587)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.