Plasma-polymerized antifouling biochips for label-free measurement of protease activity in cell culture media
DC Field | Value | Language |
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dc.contributor.author | Park, Jisoo | - |
dc.contributor.author | Kim, Gae Baik | - |
dc.contributor.author | Lippitz, Andreas | - |
dc.contributor.author | Kim, Young Mi | - |
dc.contributor.author | Jung, Donggeun | - |
dc.contributor.author | Unger, Wolfgang E. S. | - |
dc.contributor.author | Kim, Young-Pil | - |
dc.contributor.author | Lee, Tae Geol | - |
dc.date.accessioned | 2021-08-02T12:27:32Z | - |
dc.date.available | 2021-08-02T12:27:32Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/15078 | - |
dc.description.abstract | We report polyethylene glycol (PEG)-grafting antifouling surfaces using a plasma copolymerized (PcP) technique to monitor protease activity in complex media. By varying the mixing ratio of the PEG and ethylenediamine (EDA) precursors, the PcP-PEG-EDA (PcP-PE) film was able to easily control surface amine density with good preservation of the internal PEG structure. We found that nonspecific protein adsorption was dramatically reduced in serum-containing media on the PcP-PE films, as opposed to that on plasma polymerized-EDA (PP-E) films without PEG. When SPR sensor chips coated with PcP-PE film were employed to detect protease activity, biotinylated luciferase probes (luciferase-peptide-biotin) on streptavidin-conjugated SPR chips enabled real-time and label-free measurement of matrix metalloproteinase activity in cell culture media. Owing to its excellent antifouling ability, this newly developed method boasts minimal nonspecific binding and can serve as a biochip platform to promote a wide range of applications in the biological field. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | Plasma-polymerized antifouling biochips for label-free measurement of protease activity in cell culture media | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Pil | - |
dc.identifier.doi | 10.1016/j.snb.2018.10.123 | - |
dc.identifier.scopusid | 2-s2.0-85055908290 | - |
dc.identifier.wosid | 000455021500066 | - |
dc.identifier.bibliographicCitation | SENSORS AND ACTUATORS B-CHEMICAL, v.281, pp.527 - 534 | - |
dc.relation.isPartOf | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.title | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.volume | 281 | - |
dc.citation.startPage | 527 | - |
dc.citation.endPage | 534 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | MATRIX-METALLOPROTEINASE ACTIVITY | - |
dc.subject.keywordPlus | GOLD NANOPARTICLE | - |
dc.subject.keywordPlus | ENERGY-TRANSFER | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | GLYCOL) | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | COPOLYMERS | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordAuthor | Protease activity | - |
dc.subject.keywordAuthor | Plasma copolymerization | - |
dc.subject.keywordAuthor | Antifouling | - |
dc.subject.keywordAuthor | Nonspecific adsorption | - |
dc.subject.keywordAuthor | Surface plasmon resonance | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0925400518319002?via%3Dihub | - |
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