Functional roles of glutamic acid E143 and E705 residues in the N-terminus and transmembrane domain 7 of Anoctamin 1 in calcium and noxious heat sensing
DC Field | Value | Language |
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dc.contributor.author | Choi, Jonghyun | - |
dc.contributor.author | Jang, Yongwoo | - |
dc.contributor.author | Kim, Haedong | - |
dc.contributor.author | Wee, Jungwon | - |
dc.contributor.author | Cho, Sinyoung | - |
dc.contributor.author | Son, Woo Sung | - |
dc.contributor.author | Kim, Sung Min | - |
dc.contributor.author | Yang, Young Duk | - |
dc.date.accessioned | 2022-07-12T20:00:19Z | - |
dc.date.available | 2022-07-12T20:00:19Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/150879 | - |
dc.description.abstract | Anoctamin 1 (ANO1) is an anion channel that is activated by changes in cytosolic Ca²⁺ concentration and noxious heat Although the critical roles of ANO1 have been elucidated in various cell types, the control of its gating mechanisms by Ca²⁺ and heat remain more elusive. To investigate critical amino acid residues for modulation of Ca²⁺ and heat sensing, we constructed a randomized mutant library for ANO1. Among 695 random mutants, reduced Ca²⁺ sensitivity was observed in two mutants (mutant 84 and 87). Consequently, the E143A mutant showed reduced sensitivity to Ca²⁺ but not to high temperatures, whereas the E705V mutant exhibited reduced sensitivity to both Ca²⁺ and noxious heat These results suggest that the glutamic acids (E) at 143 and 705 residues in ANO1 are critical for modulation of Ca²⁺ and/or heat responses. Furthermore, these findings help to provide a better understanding of the Ca²⁺-mediated activation and heat-sensing mechanism of ANO1. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.title | Functional roles of glutamic acid E143 and E705 residues in the N-terminus and transmembrane domain 7 of Anoctamin 1 in calcium and noxious heat sensing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jang, Yongwoo | - |
dc.contributor.affiliatedAuthor | Kim, Sung Min | - |
dc.identifier.doi | 10.5483/BMBRep.2018.51.5.199 | - |
dc.identifier.scopusid | 2-s2.0-85047978050 | - |
dc.identifier.wosid | 000434114800007 | - |
dc.identifier.bibliographicCitation | BMB REPORTS, v.51, no.5, pp.236 - 241 | - |
dc.relation.isPartOf | BMB REPORTS | - |
dc.citation.title | BMB REPORTS | - |
dc.citation.volume | 51 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 236 | - |
dc.citation.endPage | 241 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002349872 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | CHLORIDE CHANNELS | - |
dc.subject.keywordPlus | SECRETORY EPITHELIA | - |
dc.subject.keywordPlus | INTRACELLULAR LOOP | - |
dc.subject.keywordPlus | TMEM16A | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CA²⁺ | - |
dc.subject.keywordAuthor | Anoctamin 1 | - |
dc.subject.keywordAuthor | Calcium sensitivity | - |
dc.subject.keywordAuthor | Heat sensitivity | - |
dc.subject.keywordAuthor | Random mutation | - |
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