Dual function of Lactobacillus kefiri DH5 in preventing high-fat-diet-induced obesity: direct reduction of cholesterol and upregulation of PPAR-α in adipose tissue
DC Field | Value | Language |
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dc.contributor.author | Kim, Dong-Hyeon | - |
dc.contributor.author | Jeong, Dana | - |
dc.contributor.author | Kang, Il-Byeong | - |
dc.contributor.author | Kim, Hyunsook | - |
dc.contributor.author | Song, Kwang-Young | - |
dc.contributor.author | Seo, Kun-Ho | - |
dc.date.accessioned | 2022-07-12T23:52:03Z | - |
dc.date.available | 2022-07-12T23:52:03Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 1613-4125 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/151239 | - |
dc.description.abstract | Scope Kefir consumption inhibits the development of obesity and non-alcoholic fatty liver disease (NALFD) in mice fed 60% high-fat diet (HFD). To identify the key contributor of this effect, we isolated lactic acid bacteria (LAB) from kefir and examined their anti-obesity properties from in vitro screening and in vivo validation. Methods and results Thirteen kefir LAB isolates were subjected to survivability test using artificial gastrointestinal environment and cholesterol-reducing assay. Lactobacillus kefiri DH5 showed 100% survivability in gastrointestinal environments and reduced 51.6% of cholesterol; thus, this strain was selected for in vivo experiment. Compared to the HFD-saline group, the HFD-DH5 group showed significantly lower body weight (34.68 versus 31.10 g; p < 0.001), epididymal adipose tissue weight (1.39 versus 1.05 g; p < 0.001), blood triglyceride (38.2 versus 31.0 mg/dL; p < 0.01) and LDL-cholesterol levels (19.4 versus 15.7 mg/dL; p < 0.01). In addition, L. kefiri DH5 administration significantly modulated gut microbiota of HFD-fed mice. The hepatic steatosis was significantly milder (Lesion score, 2.1 versus 1.2; p < 0.001) and adipocyte diameter was significantly smaller (65.1 versus 42.2 μm; p < 0.001) in the HFD-DH5 group. L. kefiri DH5 upregulated PPAR-α, FABP4, and CPT1 expression in the epididymal adipose tissues (2.29-, 1.77-, and 2.05-fold change, respectively), suggesting a reduction in adiposity by stimulating fatty acid oxidation. Conclusion L. kefiri DH5 exerts anti-obesity effects by direct reduction of cholesterol in the lumen and upregulation of PPAR-α gene in adipose tissues. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Dual function of Lactobacillus kefiri DH5 in preventing high-fat-diet-induced obesity: direct reduction of cholesterol and upregulation of PPAR-α in adipose tissue | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunsook | - |
dc.identifier.doi | 10.1002/mnfr.201700252 | - |
dc.identifier.scopusid | 2-s2.0-85032657142 | - |
dc.identifier.wosid | 000414355700009 | - |
dc.identifier.bibliographicCitation | MOLECULAR NUTRITION & FOOD RESEARCH, v.61, no.11, pp.1 - 12 | - |
dc.relation.isPartOf | MOLECULAR NUTRITION & FOOD RESEARCH | - |
dc.citation.title | MOLECULAR NUTRITION & FOOD RESEARCH | - |
dc.citation.volume | 61 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | LACTIC-ACID BACTERIA | - |
dc.subject.keywordPlus | HEPATIC STEATOSIS | - |
dc.subject.keywordPlus | GUT MICROBIOME | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ACIDOPHILUS | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Cholesterol reduction | - |
dc.subject.keywordAuthor | High fat diet | - |
dc.subject.keywordAuthor | Lactobacillus kefiri | - |
dc.subject.keywordAuthor | Obesity | - |
dc.subject.keywordAuthor | PPAR-alpha | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201700252 | - |
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