An ultra-effective method of generating extramultipotent cells from human fibroblasts by ultrasound
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Yong Seung | - |
dc.contributor.author | Heo, Hyejung | - |
dc.contributor.author | Lee, Jonghwan | - |
dc.contributor.author | Moon, Sung Ung | - |
dc.contributor.author | Jung, Woon Yong | - |
dc.contributor.author | Park, Yong Keun | - |
dc.contributor.author | Park, Min Geun | - |
dc.contributor.author | Oh, Seung-Hun | - |
dc.contributor.author | Kim, Soonhag | - |
dc.date.accessioned | 2022-07-13T04:32:12Z | - |
dc.date.available | 2022-07-13T04:32:12Z | - |
dc.date.created | 2021-05-14 | - |
dc.date.issued | 2017-10 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/151433 | - |
dc.description.abstract | Multipotent cells have similar basic features of all stem cells but limitation in ability of self-renewal and differentiation compared with pluripotent cells. Here, we have developed an ultra effective, gene- and chemical-free method of generating extra multipotent (xpotent) cells which have differentiation potential more than limited cell types, by the mechanism of ultrasound-directed permeation of environmental transition-guided cellular reprogramming (Entr). Ultrasound stimulus generated a massive number of Entr-mediated xpotent (x/Entr) spheroids from human dermal fibroblasts (HDFs) 6 days after treatment. The emergence of x/Entr was first initiated by the introduction of human embryonic stem cell (ESC) environments into the HDFs to start fast cellular reprogramming including activation of stress related kinase signaling pathways, subsequent chromatin remodeling, and expression of pluripotentrelated genes via transient membrane damage caused by ultrasound-induced cavitation. And then, pluripotent markers were transported into their adjacent HDFs via direct cell-to-cell connections in order to generate xpotent clusters. The features of x/Entr cells were intermediate between pluripotency and multipotency in terms of pluripotency with three germ layer markers, multi-lineage differentiation potential, and no teratoma formation. This physical stimulus-mediated reprogramming strategy was cost-effective, simple, quick, produced significant yields, and was safe, and can therefore provide a new paradigm for clinical application. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | An ultra-effective method of generating extramultipotent cells from human fibroblasts by ultrasound | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jung, Woon Yong | - |
dc.identifier.doi | 10.1016/j.biomaterials.2017.07.033 | - |
dc.identifier.scopusid | 2-s2.0-85026368170 | - |
dc.identifier.wosid | 000418391800006 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.143, pp.65 - 78 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 143 | - |
dc.citation.startPage | 65 | - |
dc.citation.endPage | 78 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | LOW-INTENSITY | - |
dc.subject.keywordPlus | NEURAL DIFFERENTIATION | - |
dc.subject.keywordPlus | STEM/PROGENITOR CELLS | - |
dc.subject.keywordPlus | DNA METHYLATION | - |
dc.subject.keywordPlus | EMBRYOID BODIES | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | Ultrasound | - |
dc.subject.keywordAuthor | Influx of extracellular environment | - |
dc.subject.keywordAuthor | Chromatin remodeling | - |
dc.subject.keywordAuthor | Cellular reprogramming | - |
dc.subject.keywordAuthor | Multipotent cells | - |
dc.subject.keywordAuthor | Human fibroblast | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0142961217304891?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.