Comprehensive analysis of human protein N-termini enables assessment of various protein forms
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeom, Jeonghun | - |
dc.contributor.author | Ju, Shinyeong | - |
dc.contributor.author | Choi, YunJin | - |
dc.contributor.author | Paek, Eunok | - |
dc.contributor.author | Lee, Cheolju | - |
dc.date.accessioned | 2022-07-14T00:54:05Z | - |
dc.date.available | 2022-07-14T00:54:05Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/152066 | - |
dc.description.abstract | Various forms of protein (proteoforms) are generated by genetic variations, alternative splicing, alternative translation initiation, co- or post-translational modification and proteolysis. Different proteoforms are in part discovered by characterizing their N-terminal sequences. Here, we introduce an N-terminal-peptide-enrichment method, Nrich. Filter-aided negative selection formed the basis for the use of two N-blocking reagents and two endoproteases in this method. We identified 6,525 acetylated (or partially acetylated) and 6,570 free protein N-termini arising from 5,727 proteins in HEK293T human cells. The protein N-termini included translation initiation sites annotated in the UniProtKB database, putative alternative translational initiation sites, and N-terminal sites exposed after signal/transit/propeptide removal or unknown processing, revealing various proteoforms in cells. In addition, 46 novel protein N-termini were identified in 5' untranslated region (UTR) sequence with pseudo start codons. Our data showing the observation of N-terminal sequences of mature proteins constitutes a useful resource that may provide information for a better understanding of various proteoforms in cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Comprehensive analysis of human protein N-termini enables assessment of various protein forms | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paek, Eunok | - |
dc.identifier.doi | 10.1038/s41598-017-06314-9 | - |
dc.identifier.scopusid | 2-s2.0-85026416796 | - |
dc.identifier.wosid | 000406366400007 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.7 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 7 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | ALTERNATIVE TRANSLATION INITIATION | - |
dc.subject.keywordPlus | MS-GF PLUS | - |
dc.subject.keywordPlus | DATABASE | - |
dc.subject.keywordPlus | PROTEOMICS | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | ACETYLTRANSFERASES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | SEARCH | - |
dc.subject.keywordPlus | ACETYLATION | - |
dc.subject.keywordPlus | INSIGHTS | - |
dc.identifier.url | https://www.nature.com/articles/s41598-017-06314-9 | - |
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