Genome-scale analysis of Acetobacterium bakii reveals the cold adaptation of psychrotolerant acetogens by post-transcriptional regulation
DC Field | Value | Language |
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dc.contributor.author | Shin, Jongoh | - |
dc.contributor.author | Song, Yoseb | - |
dc.contributor.author | Jin, Sangrak | - |
dc.contributor.author | Lee, Jung-Kul | - |
dc.contributor.author | Kim, Dong Rip | - |
dc.contributor.author | Kim, Sun Chang | - |
dc.contributor.author | Cho, Suhyung | - |
dc.contributor.author | Cho, Byung-Kwan | - |
dc.date.accessioned | 2021-08-02T12:29:35Z | - |
dc.date.available | 2021-08-02T12:29:35Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 1355-8382 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/15235 | - |
dc.description.abstract | Acetogens synthesize acetyl-CoA via CO₂ or CO fixation, producing organic compounds. Despite their ecological and industrial importance, their transcriptional and post-transcriptional regulation has not been systematically studied. With completion of the genome sequence of Acetobacterium bakii (4.28-Mb), we measured changes in the transcriptome of this psychrotolerant acetogen in response to temperature variations under autotrophic and heterotrophic growth conditions. Unexpectedly, acetogenesis genes were highly up-regulated at low temperatures under heterotrophic, as well as autotrophic, growth conditions. To mechanistically understand the transcriptional regulation of acetogenesis genes via changes in RNA secondary structures of 5'-untranslated regions (5'-UTR), the primary transcriptome was experimentally determined, and 1379 transcription start sites (TSS) and 1100 5'-UTR were found. Interestingly, acetogenesis genes contained longer 5'-UTR with lower RNA-folding free energy than other genes, revealing that the 5'-UTRs control the RNA abundance of the acetogenesis genes under low temperature conditions. Our findings suggest that post-transcriptional regulation via RNA conformational changes of 5'-UTRs is necessary for cold-adaptive acetogenesis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | - |
dc.title | Genome-scale analysis of Acetobacterium bakii reveals the cold adaptation of psychrotolerant acetogens by post-transcriptional regulation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Dong Rip | - |
dc.identifier.doi | 10.1261/rna.068239.118 | - |
dc.identifier.scopusid | 2-s2.0-85056570167 | - |
dc.identifier.wosid | 000450373800018 | - |
dc.identifier.bibliographicCitation | RNA, v.24, no.12, pp.1839 - 1855 | - |
dc.relation.isPartOf | RNA | - |
dc.citation.title | RNA | - |
dc.citation.volume | 24 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1839 | - |
dc.citation.endPage | 1855 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | METHANOGENIC ARCHAEA | - |
dc.subject.keywordPlus | COMMODITY CHEMICALS | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | START SITES | - |
dc.subject.keywordPlus | CSPA | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordAuthor | post-transcriptional regulation | - |
dc.subject.keywordAuthor | psychrotolerant acetogen | - |
dc.subject.keywordAuthor | Acetobacterium bakii | - |
dc.subject.keywordAuthor | cold-adaptive acetogenesis | - |
dc.identifier.url | https://rnajournal.cshlp.org/content/24/12/1839 | - |
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