Effects of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system-Based Deletion of miR-451 in Mouse Embryonic Stem Cells on Their Self-Renewal and Hematopoietic Differentiation
DC Field | Value | Language |
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dc.contributor.author | Kim, Su-Jin | - |
dc.contributor.author | Kim, Chang-Hoon | - |
dc.contributor.author | An, Borim | - |
dc.contributor.author | Ha, Kwon-Soo | - |
dc.contributor.author | Hong, Seok-Ho | - |
dc.contributor.author | Kim, Kye-Seong | - |
dc.date.accessioned | 2022-07-14T07:57:39Z | - |
dc.date.available | 2022-07-14T07:57:39Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/152599 | - |
dc.description.abstract | Pluripotent stem cells (PSCs) are a useful source of cells for exploring the role of genes related with early developmental processes and specific diseases due to their ability to differentiate into all somatic cell types. Recently, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system has proven to be a robust tool for targeted genetic modification. Here, we generated miR-451-deficient PSCs using the CRISPR/Cas9 system with PCR-based homologous recombination donor and investigated the impact of its deletion on self-renewal and hematopoietic development. CRISPR/Cas9-mediated miR-451 knockout did not alter the gene expressions of pluripotency, cellular morphology, and cell cycle, but led to impaired erythrocyte development. These findings propose that a combination of PSCs and CRISPR/Cas9 system could be useful to promote biomedical applications of PSCs by elucidating the function and manipulating of specific miRNAs during lineage specification and commitment. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | - |
dc.title | Effects of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) protein 9 system-Based Deletion of miR-451 in Mouse Embryonic Stem Cells on Their Self-Renewal and Hematopoietic Differentiation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kye-Seong | - |
dc.identifier.doi | 10.1007/s13770-017-0031-8 | - |
dc.identifier.scopusid | 2-s2.0-85017124428 | - |
dc.identifier.wosid | 000399161300011 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.14, no.2, pp.179 - 185 | - |
dc.relation.isPartOf | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.title | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.volume | 14 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 179 | - |
dc.citation.endPage | 185 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002212833 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.subject.keywordPlus | ERYTHROID-DIFFERENTIATION | - |
dc.subject.keywordPlus | CRISPR/CAS9 SYSTEM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | ERYTHROPOIESIS | - |
dc.subject.keywordPlus | RECOMBINATION | - |
dc.subject.keywordPlus | CRISPR-CAS9 | - |
dc.subject.keywordPlus | 14-3-3-ZETA | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | MIRNAS | - |
dc.subject.keywordAuthor | CRISPR/Cas9 | - |
dc.subject.keywordAuthor | Pluripotent stem cells | - |
dc.subject.keywordAuthor | MicroRNA | - |
dc.subject.keywordAuthor | Hematopoiesis | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs13770-017-0031-8 | - |
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