Nestin Expression in the Adult Mouse Retina with Pharmaceutically Induced Retinal Degeneration
DC Field | Value | Language |
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dc.contributor.author | Moon, Chan Hee | - |
dc.contributor.author | Cho, Heeyoon | - |
dc.contributor.author | Kim, Yoon Kyung | - |
dc.contributor.author | Park, Tae Kwann | - |
dc.date.accessioned | 2022-07-14T17:00:41Z | - |
dc.date.available | 2022-07-14T17:00:41Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/152949 | - |
dc.description.abstract | The present study investigated the temporal pattern and cellular localization of nestin in the adult mouse retina with pharmaceutically induced retinal degeneration using N-methyl-N-nitrosourea (MNU). After a single intraperitoneal injection of MNU in 8-weekold C57BL/6 mice, the animals were sacrificed at 1, 3, 5, 7, and 21 days (n=6, in each stage). The eyes were examined by means of immunohistochemical tests using nestin, ionized calcium-binding adaptor molecule (Iba-1), CD11b, F4/80, and glial fibrillary acidic protein (GFAP). Western blot analysis and manual cell counting were performed for quantification. Nestin expression was increased after MNU administration. Nestin+/Iba-1+ cells were migrated into outer nuclear layer (ONL) and peaked at day 3 post injection (PI). Nestin+/CD11b+ cells were also mainly identified in ONL at day 3 PI and peaked at day 5. Nestin+/F4/80+ cells were shown in the subretinal space and peaked at day 3 PI. Nestin+/GFAP+ cells were distinctly increased at day 1 PI and peaked at day 5 PI. The up-regulation of nestin expression after MNU administration in adult mouse retinal microglia, and monocyte/macrophage suggests that when retinal degeneration progresses, these cells may revert to a more developmentally immature state. Muller cells also showed reactive gliosis and differentiational changes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN ACAD MEDICAL SCIENCES | - |
dc.title | Nestin Expression in the Adult Mouse Retina with Pharmaceutically Induced Retinal Degeneration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Heeyoon | - |
dc.identifier.doi | 10.3346/jkms.2017.32.2.343 | - |
dc.identifier.scopusid | 2-s2.0-85011684810 | - |
dc.identifier.wosid | 000392204500025 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN MEDICAL SCIENCE, v.32, no.2, pp.343 - 351 | - |
dc.relation.isPartOf | JOURNAL OF KOREAN MEDICAL SCIENCE | - |
dc.citation.title | JOURNAL OF KOREAN MEDICAL SCIENCE | - |
dc.citation.volume | 32 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 343 | - |
dc.citation.endPage | 351 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002192380 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.subject.keywordPlus | MULLER GLIAL-CELLS | - |
dc.subject.keywordPlus | FIBRILLARY ACIDIC PROTEIN | - |
dc.subject.keywordPlus | RAT RETINA | - |
dc.subject.keywordPlus | REACTIVE ASTROCYTES | - |
dc.subject.keywordPlus | MARKER NESTIN | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | INCREASES | - |
dc.subject.keywordAuthor | Nestin | - |
dc.subject.keywordAuthor | N-methyl-N-nitrosourea (MNU) | - |
dc.subject.keywordAuthor | Retinal Degeneration | - |
dc.subject.keywordAuthor | Mouse | - |
dc.identifier.url | https://jkms.org/DOIx.php?id=10.3346/jkms.2017.32.2.343 | - |
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