PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Trang Thi Thu Nguyen | - |
dc.contributor.author | Park, Wei Sun | - |
dc.contributor.author | Park, Byung Ouk | - |
dc.contributor.author | Kim, Cha Yeon | - |
dc.contributor.author | Oh, Yohan | - |
dc.contributor.author | Kim, Jin Man | - |
dc.contributor.author | Choi, Hana | - |
dc.contributor.author | Kyung, Taeyoon | - |
dc.contributor.author | Kim, Cheol-Hee | - |
dc.contributor.author | Lee, Gabsang | - |
dc.contributor.author | Hahn, Klaus M. | - |
dc.contributor.author | Meyer, Tobias | - |
dc.contributor.author | Heo, Won Do | - |
dc.date.accessioned | 2022-07-15T07:08:22Z | - |
dc.date.available | 2022-07-15T07:08:22Z | - |
dc.date.created | 2021-05-14 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/153935 | - |
dc.description.abstract | Cells migrate by directing Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division control protein 42 (Cdc42) activities and by polymerizing actin toward the leading edge of the cell. Previous studies have proposed that this polarization process requires a local positive feedback in the leading edge involving Rac small GTPase and actin polymerization with PI3K likely playing a coordinating role. Here, we show that the pleckstrin homology and RhoGEF domain containing G3 (PLEKHG3) is a PI3K-regulated Rho guanine nucleotide exchange factor (RhoGEF) for Rac1 and Cdc42 that selectively binds to newly polymerized actin at the leading edge of migrating fibroblasts. Optogenetic inactivation of PLEKHG3 showed that PLEKHG3 is indispensable both for inducing and for maintaining cell polarity. By selectively binding to newly polymerized actin, PLEKHG3 promotes local Rac1/Cdc42 activation to induce more local actin polymerization, which in turn promotes the recruitment of more PLEKHG3 to induce and maintain cell front. Thus, autocatalytic reinforcement of PLEKHG3 localization to the leading edge of the cell provides a molecular basis for the proposed positive feedback loop that is required for cell polarization and directed migration. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.title | PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Yohan | - |
dc.identifier.doi | 10.1073/pnas.1604720113 | - |
dc.identifier.scopusid | 2-s2.0-84986268715 | - |
dc.identifier.wosid | 000383094500044 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.113, no.36, pp.10091 - 10096 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 113 | - |
dc.citation.number | 36 | - |
dc.citation.startPage | 10091 | - |
dc.citation.endPage | 10096 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NUCLEOTIDE EXCHANGE FACTORS | - |
dc.subject.keywordPlus | DISTINCT ROLES | - |
dc.subject.keywordPlus | RHO-GTPASES | - |
dc.subject.keywordPlus | CORONIN 1B | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | MOTILITY | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PROTRUSION | - |
dc.subject.keywordPlus | RAC | - |
dc.subject.keywordPlus | FIBROBLASTS | - |
dc.subject.keywordAuthor | PLEKHG3 | - |
dc.subject.keywordAuthor | cell polarity | - |
dc.subject.keywordAuthor | F-actin binding | - |
dc.subject.keywordAuthor | positive feedback | - |
dc.subject.keywordAuthor | PI3K | - |
dc.identifier.url | https://www.pnas.org/content/113/36/10091 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.