Functional coupling with cardiac muscle promotes maturation of hPSC-derived sympathetic neurons
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Yohan | - |
dc.contributor.author | Cho, Gun-Sik | - |
dc.contributor.author | Li, Zhe | - |
dc.contributor.author | Hong, Ingie | - |
dc.contributor.author | Zhu, Renjun | - |
dc.contributor.author | Kim, Min-Jeong | - |
dc.contributor.author | Kim, Yong Jun | - |
dc.contributor.author | Tampakakis, Emmanouil | - |
dc.contributor.author | Tung, Leslie | - |
dc.contributor.author | Huganir, Richard | - |
dc.contributor.author | Dong, Xinzhong | - |
dc.contributor.author | Kwon, Chulan | - |
dc.contributor.author | Lee, Gabsang | - |
dc.date.accessioned | 2022-07-15T14:13:39Z | - |
dc.date.available | 2022-07-15T14:13:39Z | - |
dc.date.created | 2021-05-14 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 1934-5909 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/154260 | - |
dc.description.abstract | Neurons derived from human pluripotent stem cells (hPSCs) are powerful tools for studying human neural development and diseases. Robust functional coupling of hPSC-derived neurons with target tissues in vitro is essential for modeling intercellular physiology in a dish and to further translational studies, but it has proven difficult to achieve. Here, we derive sympathetic neurons from hPSCs and show that they can form physical and functional connections with cardiac muscle cells. Using multiple hPSC reporter lines, we recapitulated human autonomic neuron development in vitro and successfully isolated PHOX2B::eGFP+ neurons that exhibit sympathetic marker expression and electrophysiological properties and norepinephrine secretion. Upon pharmacologic and optogenetic manipulation, PHOX2B:: eGFP+ neurons controlled beating rates of cardiomyocytes, and the physical interactions between these cells increased neuronal maturation. This study provides a foundation for human sympathetic neuron specification and for hPSC-based neuronal control of organs in a dish. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.title | Functional coupling with cardiac muscle promotes maturation of hPSC-derived sympathetic neurons | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Yohan | - |
dc.identifier.doi | 10.1016/j.stem.2016.05.002 | - |
dc.identifier.scopusid | 2-s2.0-85008147322 | - |
dc.identifier.wosid | 000381620500014 | - |
dc.identifier.bibliographicCitation | CELL STEM CELL, v.19, no.1, pp.95 - 106 | - |
dc.relation.isPartOf | CELL STEM CELL | - |
dc.citation.title | CELL STEM CELL | - |
dc.citation.volume | 19 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 95 | - |
dc.citation.endPage | 106 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | NEURAL CREST | - |
dc.subject.keywordPlus | NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | MORPHOLOGICAL DIVERSITY | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | SPECIFICATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1934590916300868?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.