Trichomonas vaginalis Induces IL-1 beta Production in a Human Prostate Epithelial Cell Line by Activating the NLRP3 Inflammasome Via Reactive Oxygen Species and Potassium Ion Efflux
DC Field | Value | Language |
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dc.contributor.author | Gu, Na-Yeong | - |
dc.contributor.author | Kim, Jung-Hyun | - |
dc.contributor.author | Han, Ik-Hwan | - |
dc.contributor.author | Im, Su-Jeong | - |
dc.contributor.author | Seo, Min-Young | - |
dc.contributor.author | Chung, Yong-Hoon | - |
dc.contributor.author | Ryu, Jae-Sook | - |
dc.date.accessioned | 2022-07-15T14:29:29Z | - |
dc.date.available | 2022-07-15T14:29:29Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 0270-4137 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/154292 | - |
dc.description.abstract | BACKGROUND. Trichomonas vaginalis is a sexually transmitted protozoan parasite that causes vaginitis in women, and urethritis and prostatitis in men. IL-1 beta is synthesized as immature pro-IL-1 beta, which is cleaved by activated caspase-1. Caspase-1 is, in turn, activated by a multi-protein complex known as an inflammasome. In this study, we investigated the inflammatory response of a prostate epithelial cell line (RWPE-1) to T. vaginalis and, specifically, the capacity of T. vaginalis to activate the NLRP3 inflammasome. METHODS. RWPE-1 cells were stimulated by live T. vaginalis, and subsequent expression of pro-IL-1 beta, IL-1 beta, NLRP3, ASC and caspase-1 was determined by real-time PCR and Western blotting. IL-1 beta and caspase-1 production was also measured by ELISA. To evaluate the effects of NLRP3 and caspase-1 on IL-1 beta production, the activated RWPE-1 cells were transfected with small interfering RNAs to silence the NLRP3 and caspase-1 genes. Activation of the NLRP3 inflammasome was observed by fluorescence microscopy. Intracellular reactive oxygen species (ROS) were evaluated by spectrofluorometry. RESULTS. When RWPE-1 cells were stimulated with live T. vaginalis, the mRNA and protein expression of IL-1 beta, NLRP3, ASC, and caspase-1 increased. Moreover, silencing of NLRP3 and caspase-1 attenuated T. vaginalis-induced IL-1 beta secretion. The NADPH oxidase inhibitor DPI and high extracellular potassium ion suppressed the production of IL-1 beta, caspase-1, and the expression of NLRP3 and ASC proteins. The specific NF-kappa B inhibitor, Bay 11-7082, inhibited IL-1 beta production, and also inhibited the production of caspase-1, ASC and NLRP3 proteins. CONCLUSIONS. T. vaginalis induces the formation of the NLRP3 inflammasome in human prostate epithelial cells via ROS and potassium ion efflux, and this results in IL-1 beta production. This is the first evidence for activation of the NLRP3 inflammasome in the inflammatory response by prostate epithelial cells infected with T. vaginalis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Trichomonas vaginalis Induces IL-1 beta Production in a Human Prostate Epithelial Cell Line by Activating the NLRP3 Inflammasome Via Reactive Oxygen Species and Potassium Ion Efflux | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ryu, Jae-Sook | - |
dc.identifier.doi | 10.1002/pros.23178 | - |
dc.identifier.scopusid | 2-s2.0-84960145139 | - |
dc.identifier.wosid | 000379171000002 | - |
dc.identifier.bibliographicCitation | PROSTATE, v.76, no.10, pp.885 - 896 | - |
dc.relation.isPartOf | PROSTATE | - |
dc.citation.title | PROSTATE | - |
dc.citation.volume | 76 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 885 | - |
dc.citation.endPage | 896 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | MEDIATORS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | IL-18 | - |
dc.subject.keywordAuthor | prostate epithelial cell | - |
dc.subject.keywordAuthor | Trichomonas vaginalis | - |
dc.subject.keywordAuthor | IL-1 beta | - |
dc.subject.keywordAuthor | NLRP3 inflammasome | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/pros.23178 | - |
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