Functional Restoration of Amyotrophic Lateral Sclerosis Patient-Derived Mesenchymal Stromal Cells Through Inhibition of DNA Methyltransferase
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Youn Seo | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.contributor.author | Cho, Goang-Won | - |
dc.date.accessioned | 2022-07-15T17:00:58Z | - |
dc.date.available | 2022-07-15T17:00:58Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.issn | 0272-4340 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/154690 | - |
dc.description.abstract | Alteration of DNA methylation is highly associated with aging and neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS). Remedying these aberrant methylation patterns may serve to improve these diseases. Previously, we reported that human bone marrow mesenchymal stromal cells isolated from ALS patients (ALS-MSCs) have functionally decreased stem cell potency, and excessively express DNA methyltransferases (DNMTs). In this study, we examined the correlation between excessive DNMT expression and functional decline in ALS-MSCs. The DNMT inhibitor RG108 was used for this. RG108-treated ALS-MSCs exhibit increased expression of the anti-senescence genes TERT, VEGF, and ANG, and decreased expression of the senescence-related genes ATM and p21. The activity of SA-beta-galactosidase and the expression of senescence proteins p53 and p16 were reduced in RG108-treated ALS-MSCs. The abilities of cell migration and protection against oxidative damage were improved in the treated ALS-MSCs. In neuronal differentiation experiments, the treated MSCs more effectively differentiated into neuron-like cells. These results suggest that ALS-MSC function can be restored by inhibiting excessively expressed DNMTs, an approach that may ultimately provide better efficacy in stem cell therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER/PLENUM PUBLISHERS | - |
dc.title | Functional Restoration of Amyotrophic Lateral Sclerosis Patient-Derived Mesenchymal Stromal Cells Through Inhibition of DNA Methyltransferase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Seung Hyun | - |
dc.identifier.doi | 10.1007/s10571-015-0242-2 | - |
dc.identifier.scopusid | 2-s2.0-84937923516 | - |
dc.identifier.wosid | 000376466100013 | - |
dc.identifier.bibliographicCitation | CELLULAR AND MOLECULAR NEUROBIOLOGY, v.36, no.4, pp.613 - 620 | - |
dc.relation.isPartOf | CELLULAR AND MOLECULAR NEUROBIOLOGY | - |
dc.citation.title | CELLULAR AND MOLECULAR NEUROBIOLOGY | - |
dc.citation.volume | 36 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 613 | - |
dc.citation.endPage | 620 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | PROMOTES NEURONAL DIFFERENTIATION | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | METHYLATION | - |
dc.subject.keywordPlus | HYPERMETHYLATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | RG108 | - |
dc.subject.keywordAuthor | Bone marrow mesenchymal stromal cells (BM-MSCs) | - |
dc.subject.keywordAuthor | Amyotrophic lateral sclerosis (ALS) | - |
dc.subject.keywordAuthor | DNA methyltransferases (DNMTs) | - |
dc.subject.keywordAuthor | DNMT inhibition | - |
dc.subject.keywordAuthor | RG108 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs10571-015-0242-2 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.