Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
DC Field | Value | Language |
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dc.contributor.author | Yoo, Chae-Kyung | - |
dc.contributor.author | Jeon, Jae-Yun | - |
dc.contributor.author | Kim, You-Jin | - |
dc.contributor.author | Kim, Seong-Gon | - |
dc.contributor.author | Hwang, Kyung Gyun | - |
dc.date.accessioned | 2022-07-15T18:06:16Z | - |
dc.date.available | 2022-07-15T18:06:16Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 2288-8101 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/154938 | - |
dc.description.abstract | Background: The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane forguided bone regeneration (GBR). Methods: Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cellscultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, theattachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy(SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay,and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining. Results: Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-dayobservation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (controlgroup) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly differentbetween the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane groupthan in the control group at 7 days (P < 0.05). Conclusions: These results suggest that silk fibroin is a biocompatible material that could be used as a suitablealternative barrier membrane for GBR. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한악안면성형재건외과학회 | - |
dc.title | Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Kyung Gyun | - |
dc.identifier.doi | 10.1186/s40902-016-0062-4 | - |
dc.identifier.scopusid | 2-s2.0-84983597213 | - |
dc.identifier.bibliographicCitation | Maxillofacial Plastic and Reconstructive Surgery, v.38, pp.1 - 6 | - |
dc.relation.isPartOf | Maxillofacial Plastic and Reconstructive Surgery | - |
dc.citation.title | Maxillofacial Plastic and Reconstructive Surgery | - |
dc.citation.volume | 38 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 6 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART002096977 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | Bone regeneration | - |
dc.subject.keywordAuthor | Cell adhesion | - |
dc.subject.keywordAuthor | Cell proliferation | - |
dc.subject.keywordAuthor | Membrane | - |
dc.subject.keywordAuthor | Osteoblasts | - |
dc.subject.keywordAuthor | Silk fibroin | - |
dc.identifier.url | https://jkamprs.springeropen.com/articles/10.1186/s40902-016-0062-4 | - |
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