Prediction of Putative Resistance Islands in a Carbapenem-Resistant Acinetobacter baumannii Global Clone 2 Clinical Isolate
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Yangsoon | - |
dc.contributor.author | D'Souza, Roshan | - |
dc.contributor.author | Yong, Dongeun | - |
dc.contributor.author | Lee, Kyungwon | - |
dc.date.accessioned | 2022-07-15T19:36:04Z | - |
dc.date.available | 2022-07-15T19:36:04Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/155525 | - |
dc.description.abstract | Background: We investigated the whole genome sequence (WGS) of a carbapenem-resistant Acinetobacter baumannii isolate belonging to the global clone 2 (GC2) and predicted resistance islands using a software tool. Methods: A. baumannii strain YU-R612 was isolated from the sputum of a 61-yr-old man with sepsis. The WGS of the YU-R612 strain was obtained by using the PacBio RS II Sequencing System (Pacific Biosciences Inc., USA). Antimicrobial resistance genes and resistance islands were analyzed by using ResFinder and Genomic Island Prediction software (GIPSy), respectively. Results: The YU-R612 genome consisted of a circular chromosome (ca. 4,075 kb) and two plasmids (ca. 74 kb and 5 kb). Its sequence type (ST) under the Oxford scheme was ST191, consistent with assignment to GC2. ResFinder analysis showed that YU-R612 possessed the following resistance genes: four beta-lactamase genes bla(ADC-30), bla(OXA-66), bla(OXA-23), and bla(TEM-1); armA, aadA1, and aacA4 as aminoglycoside resistance-encoding genes; aac(6')lb-cr for fluoroquinolone resistance; msr(E) for macrolide, lincosamide, and streptogramin B resistance; catB8 for phenicol resistance; and sul1 for sulfonamide resistance. By GIPSy analysis, six putative resistant islands (PRIs) were determined on the YU-R612 chromosome. Among them, PRI1 possessed two copies of Tn2009 carrying bla(OXA-23), and PRI5 carried two copies of a class I integron carrying sul1 and armA genes. Conclusions: By prediction of resistance islands in the carbapenem-resistant A. baumannii YU-R612 GC2 strain isolated in Korea, PRIs were detected on the chromosome that possessed Tn2009 and class I integrons. The prediction of resistance islands using software tools was useful for analysis of the WGS. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC LABORATORY MEDICINE | - |
dc.title | Prediction of Putative Resistance Islands in a Carbapenem-Resistant Acinetobacter baumannii Global Clone 2 Clinical Isolate | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Yangsoon | - |
dc.identifier.doi | 10.3343/alm.2016.36.4.320 | - |
dc.identifier.scopusid | 2-s2.0-84969218911 | - |
dc.identifier.wosid | 000376049300005 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, v.36, no.4, pp.320 - 324 | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.title | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.volume | 36 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 320 | - |
dc.citation.endPage | 324 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002120904 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.subject.keywordPlus | ABAR4 REPLACES ABAR3 | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | VARIANTS | - |
dc.subject.keywordPlus | SPREAD | - |
dc.subject.keywordPlus | KOREA | - |
dc.subject.keywordAuthor | Acinetobacter baumannii | - |
dc.subject.keywordAuthor | Whole genome sequence | - |
dc.subject.keywordAuthor | GIPSy | - |
dc.subject.keywordAuthor | Global clone 2 | - |
dc.identifier.url | https://www.annlabmed.org/journal/view.html?doi=10.3343/alm.2016.36.4.320 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.