The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment
DC Field | Value | Language |
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dc.contributor.author | Jeong, Eun Ju | - |
dc.contributor.author | Choi, Moonhwan | - |
dc.contributor.author | Lee, Jangwook | - |
dc.contributor.author | Rhim, Taiyoun | - |
dc.contributor.author | Lee, Kuen Yong | - |
dc.date.accessioned | 2022-07-15T19:54:58Z | - |
dc.date.available | 2022-07-15T19:54:58Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/155665 | - |
dc.description.abstract | Although chitosan and its derivatives have been frequently utilized as delivery vehicles for small interfering RNA (siRNA), it is challenging to improve the gene silencing efficiency of chitosan-based nanoparticles. In this study, we hypothesized that controlling the spacer arm length between a cell-penetrating peptide (CPP) and a nanoparticle could be critical to enhancing the cellular uptake as well as the gene silencing efficiency of conventional chitosan/siRNA nanoparticles. A peptide consisting of nine arginine units (R-9) was used as a CPP, and the spacer arm length was controlled by varying the number of glycine units between the peptide (R(9)G(n)) and the nanoparticle (n = 0, 4, and 10). Various physicochemical characteristics of R(9)G(n)-chitosan/siRNA nanoparticles were investigated in vitro. Increasing the spacing arm length did not significantly affect the complex formation between R(9)G(n)-chitosan and siRNA. However, R(9)G(10)-chitosan was much more effective in delivering genes both in vitro and in vivo compared with nonmodified chitosan (without the peptide) and R-9-chitosan (without the spacer arm). Chitosan derivatives modified with oligoarginine containing a spacer arm can be considered as potential delivery vehicles for various genes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Rhim, Taiyoun | - |
dc.contributor.affiliatedAuthor | Lee, Kuen Yong | - |
dc.identifier.doi | 10.1039/c5nr06903c | - |
dc.identifier.scopusid | 2-s2.0-84948667618 | - |
dc.identifier.wosid | 000365530700029 | - |
dc.identifier.bibliographicCitation | NANOSCALE, v.7, no.47, pp.20095 - 20104 | - |
dc.relation.isPartOf | NANOSCALE | - |
dc.citation.title | NANOSCALE | - |
dc.citation.volume | 7 | - |
dc.citation.number | 47 | - |
dc.citation.startPage | 20095 | - |
dc.citation.endPage | 20104 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | SIRNA DELIVERY | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | RNA INTERFERENCE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ARGININE | - |
dc.subject.keywordPlus | PROGRESS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | VECTORS | - |
dc.subject.keywordPlus | POLYMER | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2015/NR/C5NR06903C | - |
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