High level of interleukin-32 gamma in the joint of ankylosing spondylitis is associated with osteoblast differentiation
DC Field | Value | Language |
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dc.contributor.author | Lee, Eun-Ju | - |
dc.contributor.author | Lee, Eun-Jin | - |
dc.contributor.author | Chung, Yeon-Ho | - |
dc.contributor.author | Song, Da-Hyun | - |
dc.contributor.author | Hong, Seokchan | - |
dc.contributor.author | Lee, Chang-Keun | - |
dc.contributor.author | Yoo, Bin | - |
dc.contributor.author | Kim, Tae-Hwan | - |
dc.contributor.author | Park, Ye-Soo | - |
dc.contributor.author | Kim, Soo-Hyun | - |
dc.contributor.author | Chang, Eun-Ju | - |
dc.contributor.author | Kim, Yong-Gil | - |
dc.date.accessioned | 2022-07-15T20:05:44Z | - |
dc.date.available | 2022-07-15T20:05:44Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 1478-6354 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/155782 | - |
dc.description.abstract | Backgound: The formation of bony spurs and ankylosis is a key pathognomic feature in ankylosing spondylitis (AS) and results in functional impairment. The aim of this study was to investigate the role of IL-32 gamma in osteoblast (OB) differentiation and its association with the pathogenesis of AS. Methods: The concentration and expression of IL-32 gamma were evaluated in synovial fluid and tissue from patients with AS, rheumatoid arthritis (RA) and osteoarthritis (OA), using enzyme-linked immunosorbent assay and immunohistochemistry. To establish whether IL-32 gamma affects OB differentiation, we used calvarial cells of IL-32 gamma transgenic (TG) mice or wild-type (WT) mice. To elucidate the mechanism of osteoblastogenesis, levels of regulators were assayed in IL-32 gamma TG mice and in primary OBs after IL-32 gamma stimulation. Results: The IL-32 gamma levels were higher in the synovial fluid of AS patients compared with RA or OA patients and the expression of IL-32 was higher in AS synovia than in RA or OA synovia. Additional IL-32 gamma stimulation in precursor cells enhanced OB differentiation potentially and IL-32 gamma TG mice showed higher rates of OB differentiation than WT mice. IL-32 gamma reduced the expression of DKK-1, a negative regulator, in both WT precursor cells and human OBs and the constitutive expression of DKK-1 was suppressed in calvarial cells from IL-32 gamma TG mice. Conclusions: The elevated level of IL-32 gamma in AS joint could enhance OB differentiation via DKK-1 suppression. Therefore, IL-32 gamma might be a putative molecular target to prevent the abnormal bone formation in AS. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | High level of interleukin-32 gamma in the joint of ankylosing spondylitis is associated with osteoblast differentiation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae-Hwan | - |
dc.contributor.affiliatedAuthor | Park, Ye-Soo | - |
dc.identifier.doi | 10.1186/s13075-015-0870-4 | - |
dc.identifier.scopusid | 2-s2.0-84952877229 | - |
dc.identifier.wosid | 000366359300001 | - |
dc.identifier.bibliographicCitation | ARTHRITIS RESEARCH & THERAPY, v.17, pp.1 - 9 | - |
dc.relation.isPartOf | ARTHRITIS RESEARCH & THERAPY | - |
dc.citation.title | ARTHRITIS RESEARCH & THERAPY | - |
dc.citation.volume | 17 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Rheumatology | - |
dc.relation.journalWebOfScienceCategory | Rheumatology | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | RADIOGRAPHIC PROGRESSION | - |
dc.subject.keywordPlus | DISEASE-ACTIVITY | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | CRITERIA | - |
dc.subject.keywordPlus | IL-32 | - |
dc.subject.keywordPlus | CLASSIFICATION | - |
dc.subject.keywordPlus | OSTEOCLASTS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordAuthor | Interleukin-32 | - |
dc.subject.keywordAuthor | Osteoblast differentiation | - |
dc.subject.keywordAuthor | Ankylosing spondylitis | - |
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