Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals
DC Field | Value | Language |
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dc.contributor.author | Chang, Mi-Yoon | - |
dc.contributor.author | Oh, Boram | - |
dc.contributor.author | Rhee, Yong-Hee | - |
dc.contributor.author | Lee, Sang-Hun | - |
dc.date.accessioned | 2022-07-15T20:24:11Z | - |
dc.date.available | 2022-07-15T20:24:11Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-11 | - |
dc.identifier.issn | 1873-5061 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/156013 | - |
dc.description.abstract | Culturing human embryonic stem and induced pluripotent stem cells (hESCs/iPSCs) is one of the most costly and labor-intensive tissue cultures, as media containing expensive factors/cytokines should be changed every day to maintain and propagate undifferentiated hESCs/iPSCs in vitro. We recently reported that doxycycline, an antibacterial agent, had dramatic effects on hESC/iPSC survival and promoted self-renewal. In this study, we extended the effects of doxycycline to a more practical issue to save cost and labor in hESC/iPSC cultures. Regardless of cultured cell conditions, hESCs/iPSCs in doxycycline-supplemented media were viable and proliferating for at least 3 days without media change, while none or few viable cells were detected in the absence of doxycycline in the same conditions. Thus, hESCs/iPSCs supplemented with doxycycline can be cultured for a long period of time with media changes at 3-day intervals without altering their self-renewal and pluripotent properties, indicating that doxycycline supplementation can reduce the frequency of media changes and the amount of media required by 1/3. These findings strongly encourage the use of doxycycline to save cost and labor in culturing hESCs/iPSCs. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hun | - |
dc.identifier.doi | 10.1016/j.scr.2015.10.007 | - |
dc.identifier.scopusid | 2-s2.0-84945120480 | - |
dc.identifier.wosid | 000369036400020 | - |
dc.identifier.bibliographicCitation | STEM CELL RESEARCH, v.15, no.3, pp.608 - 613 | - |
dc.relation.isPartOf | STEM CELL RESEARCH | - |
dc.citation.title | STEM CELL RESEARCH | - |
dc.citation.volume | 15 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 608 | - |
dc.citation.endPage | 613 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | STEM-CELL LINES | - |
dc.subject.keywordPlus | HUMAN BLASTOCYSTS | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordAuthor | Human embryonic stem cell | - |
dc.subject.keywordAuthor | Survival | - |
dc.subject.keywordAuthor | Maintenance | - |
dc.subject.keywordAuthor | Doxycycline | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1873506115001427?via%3Dihub | - |
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