Effect of immobilized collagen type IV on biological properties of endothelial cells for the enhanced endothelialization of synthetic vascular graft materials
DC Field | Value | Language |
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dc.contributor.author | Heo, Yunhoe | - |
dc.contributor.author | Shin, Young Min | - |
dc.contributor.author | Lee, Yu Bin | - |
dc.contributor.author | Lim, Youn Mook | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.date.accessioned | 2022-07-15T20:50:07Z | - |
dc.date.available | 2022-07-15T20:50:07Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/156234 | - |
dc.description.abstract | Regeneration of healthy endothelium onto vascular graft materials is imperative for prevention of intimal hyperplasia and thrombogenesis. In this study, we investigated the effect of collagen type IV (COL-IV) immobilized onto electrospun nanofibers on modulation of endothelial cell (EC) function, as a potential signal to rapid endothelialization of vascular grafts. COL-IV is assembled in basement membrane underneath intimal layer and regulates morphogenesis of blood vessels. For immobilization of COL-IV, poly(L-lactic acid) (PLLA) nanofibers (PL) were prepared as a model vascular graft substrate, onto which acrylic acid (AAc) was then grafted by using gamma-ray irradiation. AAc graft was dependent on irradiation doses and AAc concentrations, which allowed us to select the condition of 5% (v/v) AAc and 10 kGy for further conjugation of COL-IV. COL-IV immobilization was proportionally controlled as a function of its concentration. Atomic force microscope (AFM) analysis qualitatively supported immobilization of COL-IV, demonstrating increase in root mean square roughness of the PL from 665.37 +/- 13.20 nm to 1440.74 +/- 33.24. However, the Young's modulus of nanofibers was retained as approximately 1 MPa, regardless of surface modification. The number of ECs attached on the nanofibers with immobilized COLIV was significantly increased by 5 times (1052 +/- 138 cells/mm(2)) from pristine PL (2341 +/- 41 cells/mm(2)). In addition, the effect of immobilized COL-IV was profound for enhancing proliferation and up-regulation of markers implicated in rapid endothelialization. Collectively, our results suggest that COL-IV immobilized onto electrospun PLLA nanofibers may serve as a promising instructive cue used in vascular graft materials. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Effect of immobilized collagen type IV on biological properties of endothelial cells for the enhanced endothelialization of synthetic vascular graft materials | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Heungsoo | - |
dc.identifier.doi | 10.1016/j.colsurfb.2015.07.003 | - |
dc.identifier.scopusid | 2-s2.0-84937205936 | - |
dc.identifier.wosid | 000362142000024 | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.134, pp.196 - 203 | - |
dc.relation.isPartOf | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 134 | - |
dc.citation.startPage | 196 | - |
dc.citation.endPage | 203 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | ARTERY-BYPASS SURGERY | - |
dc.subject.keywordPlus | SURFACE MODIFICATION | - |
dc.subject.keywordPlus | POLY(L-LACTIC ACID) | - |
dc.subject.keywordPlus | ACRYLIC-ACID | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | ADHERENCE | - |
dc.subject.keywordAuthor | Electrospinning | - |
dc.subject.keywordAuthor | Surface modification | - |
dc.subject.keywordAuthor | Endothelialization | - |
dc.subject.keywordAuthor | Vascular graft | - |
dc.subject.keywordAuthor | Collagen type IV | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0927776515300370?via%3Dihub | - |
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