Gα12 overexpressed in hepatocellular carcinoma reduces microRNA-122 expression via HNF4α inactivation, which causes c-Met induction
DC Field | Value | Language |
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dc.contributor.author | Yang, Yoon Mee | - |
dc.contributor.author | Lee, Chan Gyu | - |
dc.contributor.author | Koo, Ja Hyun | - |
dc.contributor.author | Kim, Tae Hyun | - |
dc.contributor.author | Lee, Jung Min | - |
dc.contributor.author | An, Ji hyun | - |
dc.contributor.author | Kim, Kang Mo | - |
dc.contributor.author | Kim, Sang Geon | - |
dc.date.accessioned | 2022-07-15T21:28:44Z | - |
dc.date.available | 2022-07-15T21:28:44Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2015-08 | - |
dc.identifier.issn | 19492553 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/156564 | - |
dc.description.abstract | MicroRNA-122 (miR-122) is implicated as a regulator of physiological and pathophysiological processes in the liver. Overexpression of G alpha(12) is associated with overall survival in patients with hepatocellular carcinoma (HCC). Array-based miRNA profiling was performed on Huh7 stably transfected with activated G alpha(12) to find miRNAs regulated by the G alpha(12) pathway; among them, miR-122 was most greatly repressed. miR-122 directly inhibits c-Met expression, playing a role in HCC progression. G alpha(12) destabilized HNF4 alpha by accelerating ubiquitination, impeding constitutive expression of miR-122. miR-122 mimic transfection diminished the ability of G alpha(12) to increase c-Met and to activate ERK, STAT3, and Akt/mTOR, suppressing cell proliferation with augmented apoptosis. Consistently, miR-122 transfection prohibited tumor cell colony formation and endothelial tube formation. In a xenograft model, G alpha(12) knockdown attenuated c-Met expression by restoring HNF4 alpha levels, and elicited tumor cell apoptosis but diminished Ki67 intensities. In human HCC samples, G alpha(12) levels correlated to c-Met and were inversely associated with miR-122. Both miR-122 and c-Met expression significantly changed in tumor node metastasis (TNM) stage II/III tumors. Moreover, changes in G alpha(12) and miR-122 levels discriminated recurrence-free and overall survival rates of HCC patients. Collectively, G alpha(12) overexpression in HCC inhibits MIR122 transactivation by inactivating HNF4 alpha, which causes c-Met induction, contributing to cancer aggressiveness. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | IMPACT JOURNALS LLC | - |
dc.title | Gα12 overexpressed in hepatocellular carcinoma reduces microRNA-122 expression via HNF4α inactivation, which causes c-Met induction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | An, Ji hyun | - |
dc.identifier.doi | 10.18632/oncotarget.3957 | - |
dc.identifier.scopusid | 2-s2.0-84938885005 | - |
dc.identifier.wosid | 000359360000030 | - |
dc.identifier.bibliographicCitation | ONCOTARGET, v.6, no.22, pp.19055 - 19069 | - |
dc.relation.isPartOf | ONCOTARGET | - |
dc.citation.title | ONCOTARGET | - |
dc.citation.volume | 6 | - |
dc.citation.number | 22 | - |
dc.citation.startPage | 19055 | - |
dc.citation.endPage | 19069 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | PROTEIN-COUPLED RECEPTORS | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | MESENCHYMAL TRANSITION | - |
dc.subject.keywordPlus | MIR-122 EXPRESSION | - |
dc.subject.keywordPlus | GEP ONCOGENE | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | TRANSACTIVATION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | THROMBIN | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordAuthor | liver cancer | - |
dc.subject.keywordAuthor | non-coding RNA | - |
dc.subject.keywordAuthor | G protein | - |
dc.subject.keywordAuthor | c-Met | - |
dc.identifier.url | https://www.oncotarget.com/article/3957/text/ | - |
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