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Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B 분리
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | 조아영 | - |
| dc.contributor.author | 김기영 | - |
| dc.contributor.author | 임종국 | - |
| dc.contributor.author | 모창연 | - |
| dc.contributor.author | 문지혜 | - |
| dc.contributor.author | 박샛별 | - |
| dc.contributor.author | 박수희 | - |
| dc.contributor.author | 엄애선 | - |
| dc.date.accessioned | 2022-07-15T22:53:53Z | - |
| dc.date.available | 2022-07-15T22:53:53Z | - |
| dc.date.created | 2021-05-13 | - |
| dc.date.issued | 2015-05 | - |
| dc.identifier.issn | 1226-4768 | - |
| dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/157265 | - |
| dc.description.abstract | The aim of this study is to develop a method to separate and purify the Staphylococcal enterotoxin B (SEB). The method was based on fast protein liquid chromatography (FPLC) along with gel filtration (GF) after cation exchange chromatography (CIEX) treatment. Various columns such as CM sepharose HP, Cellufine C-500, WorkBeads 40S for CIEX, and GF on Sephacryl S-100 for GF were compared in terms of separation efficiency. Consequently, Work- Beads 40S along with Sephacryl S-100 produced best separation performance. Separated SEB with developed method was verified with ELISA assay. Purified SEB with the method can be used to manufacture rapid detection kit or antibodies. | - |
| dc.language | 한국어 | - |
| dc.language.iso | ko | - |
| dc.publisher | 한국산업식품공학회 | - |
| dc.title | Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B 분리 | - |
| dc.title.alternative | Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography | - |
| dc.type | Article | - |
| dc.contributor.affiliatedAuthor | 엄애선 | - |
| dc.identifier.doi | 10.13050/foodengprog.2015.19.2.111 | - |
| dc.identifier.bibliographicCitation | 산업식품공학, v.19, no.2, pp.111 - 116 | - |
| dc.relation.isPartOf | 산업식품공학 | - |
| dc.citation.title | 산업식품공학 | - |
| dc.citation.volume | 19 | - |
| dc.citation.number | 2 | - |
| dc.citation.startPage | 111 | - |
| dc.citation.endPage | 116 | - |
| dc.type.rims | ART | - |
| dc.identifier.kciid | ART001992048 | - |
| dc.description.journalClass | 2 | - |
| dc.description.isOpenAccess | N | - |
| dc.description.journalRegisteredClass | kci | - |
| dc.subject.keywordAuthor | staphylococcal enterotoxin B (SEB) | - |
| dc.subject.keywordAuthor | fast protein liquid chromatography (FPLC) | - |
| dc.subject.keywordAuthor | separation | - |
| dc.subject.keywordAuthor | purification | - |
| dc.subject.keywordAuthor | detection | - |
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Collections - 서울 생활과학대학 > 서울 식품영양학과 > 1. Journal Articles
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Related Researcher
- Om, Ae Son
- COLLEGE OF HUMAN ECOLOGY (DEPARTMENT OF FOOD & NUTRITION)