Delivery of anti-microRNA-21 antisense-oligodeoxynucleotide using amphiphilic peptides for glioblastoma gene therapy
DC Field | Value | Language |
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dc.contributor.author | Song, Hojung | - |
dc.contributor.author | Oh, Binna | - |
dc.contributor.author | Choi, Manbok | - |
dc.contributor.author | Oh, Jungju | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-07-15T22:58:18Z | - |
dc.date.available | 2022-07-15T22:58:18Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-05 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/157309 | - |
dc.description.abstract | Inhibition of microRNA-21 (miR-21) has been shown to promote apoptosis of cancer cells and to reduce tumor size in glioblastoma. However, efficient carriers for antisense-oligodeoxynucleotide (antisense-ODN) against miR-21 have not yet been developed. In this study, the R3V6 peptide (R3V6) was evaluated as a carrier of antisense-ODN. In a gel retardation assay, R3V6 formed a complex with an antisense-ODN. The serum stability assay showed that R3V6 protected it from nucleases more efficiently than polyethylenimine (PEI; 25 kDa, PEI25k). A Renilla luciferase gene with a 3'-untranslated region (3'-UTR) recognizable by miR-21 (psiCHECK2-miR-21-UTR) was constructed for the antisense-ODN assay. psiCHECK2-miR-21-UTR expressed less Renilla luciferase in the cells with a higher level of miR-21 due to the effect of miR-21. In an in vitro transfection assay, the R3V6 peptide delivered anti-miR-21 antisense-ODN into cells more efficiently than PEI (25 kDa, PEI25k) and lipofectamine. As a result, antisense-ODN/R3V6 complex inhibited miR-21 and increased Renilla luciferase expression more efficiently than antisense-ODN/PEI25k or antisense-ODN/Lipofectamine complexes in both C6 and A172 glioblastoma cells. Furthermore, the antisense-ODN/R3V6 complexes reduced the level of miR-21 and induced apoptosis of glioblastoma cells. These results suggest that the R3V6 peptide may be a useful carrier of antisense-ODN for glioblastoma gene therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | Delivery of anti-microRNA-21 antisense-oligodeoxynucleotide using amphiphilic peptides for glioblastoma gene therapy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.3109/1061186X.2014.1000336 | - |
dc.identifier.scopusid | 2-s2.0-84927749135 | - |
dc.identifier.wosid | 000352857100008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DRUG TARGETING, v.23, no.4, pp.360 - 370 | - |
dc.relation.isPartOf | JOURNAL OF DRUG TARGETING | - |
dc.citation.title | JOURNAL OF DRUG TARGETING | - |
dc.citation.volume | 23 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 360 | - |
dc.citation.endPage | 370 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR GENE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | RNA INTERFERENCE | - |
dc.subject.keywordPlus | MICROPROCESSOR COMPLEX | - |
dc.subject.keywordPlus | MICRORNA-21 | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Amphiphilic peptide | - |
dc.subject.keywordAuthor | antisense-oligonucleotide | - |
dc.subject.keywordAuthor | gene therapy | - |
dc.subject.keywordAuthor | glioblastoma | - |
dc.subject.keywordAuthor | microRNA-21 | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.3109/1061186X.2014.1000336 | - |
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