Real-time analysis of chemotactic motion of Euglena cells confined in a microchip toxicity sensor
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ozasa, Kazunari | - |
dc.contributor.author | Lee, Jee Soo | - |
dc.contributor.author | Song, Simon | - |
dc.contributor.author | Maeda, Mizuo | - |
dc.date.accessioned | 2022-07-16T00:12:37Z | - |
dc.date.available | 2022-07-16T00:12:37Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 1013-9826 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/157833 | - |
dc.description.abstract | We developed the real-time analysis of chemotactic motion of microbial cells (Euglena gracilis), for on-chip cytotoxicity sensing for environmental chemical substances. The Euglena cells were confined in a closed-type micro-aquarium in a PDMS microchip, and their movements were taken by a CMOS video camera. When 1.5%-H2O2 was introduced into a microchannel running aside of the micro-aquarium, the H2O2 molecules permeated into the micro-aquarium by diffusion through porous PDMS wall, and the cells fell into continuous rotation instead of single step turning and/or straightforward swimming. Such an abnormal swimming behavior is the result of metabolic disturbing effects evoked by radical oxygen species released from H2O2. In order to sensing the metabolic disturbing effects, we achieved real-time categorization of the swimming traces into straightforward swimming or continuous rotation; firstly the swimming traces in the video image were sectioned into squares, and then the aspect ratio and filling factor for each square were calculated. High aspect ratio or small filling factor corresponded to straightforward swimming, whereas low aspect ratio and high filling factor to continuous rotation. This motion analysis enables to measure the metabolic disturbing effects on swimming Euglena cells quantitatively, which is important to detect unidentified toxic substances in environments. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Trans Tech Publications Ltd | - |
dc.title | Real-time analysis of chemotactic motion of Euglena cells confined in a microchip toxicity sensor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Simon | - |
dc.identifier.doi | 10.4028/www.scientific.net/KEM.644.185 | - |
dc.identifier.scopusid | 2-s2.0-84930177012 | - |
dc.identifier.bibliographicCitation | Key Engineering Materials, v.644, pp.185 - 188 | - |
dc.relation.isPartOf | Key Engineering Materials | - |
dc.citation.title | Key Engineering Materials | - |
dc.citation.volume | 644 | - |
dc.citation.startPage | 185 | - |
dc.citation.endPage | 188 | - |
dc.type.rims | ART | - |
dc.type.docType | Conference Paper | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | Aspect ratio | - |
dc.subject.keywordPlus | Biochemistry | - |
dc.subject.keywordPlus | Chemical sensors | - |
dc.subject.keywordPlus | Filling | - |
dc.subject.keywordPlus | Metabolism | - |
dc.subject.keywordPlus | Microchannels | - |
dc.subject.keywordPlus | Microprocessor chips | - |
dc.subject.keywordPlus | Motion analysis | - |
dc.subject.keywordPlus | Transducers | - |
dc.subject.keywordPlus | Video cameras | - |
dc.subject.keywordPlus | Chemical sensing | - |
dc.subject.keywordPlus | Chemotaxis | - |
dc.subject.keywordPlus | CMOS video cameras | - |
dc.subject.keywordPlus | Continuous rotation | - |
dc.subject.keywordPlus | Environmental chemicals | - |
dc.subject.keywordPlus | Euglena gracilis | - |
dc.subject.keywordPlus | Radical oxygen species | - |
dc.subject.keywordPlus | Real-time motion | - |
dc.subject.keywordPlus | Chemical analysis | - |
dc.subject.keywordAuthor | Chemical sensing | - |
dc.subject.keywordAuthor | Chemotaxis | - |
dc.subject.keywordAuthor | Euglena gracilis | - |
dc.subject.keywordAuthor | Microchannels | - |
dc.subject.keywordAuthor | Real-time motion analysis | - |
dc.identifier.url | https://www.scientific.net/KEM.644.185 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.