Enhanced electrochemical sensitivity of enzyme precipitate coating (EPC)-based glucose oxidase biosensors with increased free CNT loadings
DC Field | Value | Language |
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dc.contributor.author | Kim, Jae Hyun | - |
dc.contributor.author | Jun, Sun-Ae | - |
dc.contributor.author | Kwon, Yongchai | - |
dc.contributor.author | Ha, Su | - |
dc.contributor.author | Sang, Byong-In | - |
dc.contributor.author | Kim, Jungbae | - |
dc.date.accessioned | 2022-07-16T00:31:13Z | - |
dc.date.available | 2022-07-16T00:31:13Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 1567-5394 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/157958 | - |
dc.description.abstract | Enzymatic electrodes were fabricated by using three different immobilizations of glucose oxidase (GOX): covalent enzyme attachment (CA), enzyme coating (EC), and enzyme precipitate coating (EPC), here referred to as CA-E, EC-E, and EPC-E, respectively. When additional carbon nanotubes (CNTs) were introduced from 0 to 75 wt% for the EPC-E design, its initial biosensor sensitivity was improved from 2.40 x 10(-3) to 16.26 x 10(-3) A.M-1.cm(-2), while its electron charge transfer rate constant was increased from 0.33 to 1.47 s(-1). When a fixed ratio of CNTs was added for three different electrode systems, EPC-E showed the best glucose sensitivity and long-term thermal stability. For example, when 75 wt% of additional CNTs was added, the initial sensitivity of EPC-E was 16.26 x 10(-3) A.M-1.cm(-2), while those of EC-E and CA-E were only 6.42 x 10(-3) and 1.18 x 10(-3) A.M-1.cm(-2), respectively. Furthermore, EPC-E retained 63% of its initial sensitivity after thermal treatment at 40 degrees C over 41 days, while EC-E and CA-E showed only 12% and 1% of initial sensitivities, respectively. Consequently, the EPC approach with additional CNTs achieved both high sensitivity and long-term stability, which are required for continuous and accurate glucose monitoring. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | Enhanced electrochemical sensitivity of enzyme precipitate coating (EPC)-based glucose oxidase biosensors with increased free CNT loadings | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sang, Byong-In | - |
dc.identifier.doi | 10.1016/j.bioelechem.2014.08.017 | - |
dc.identifier.scopusid | 2-s2.0-84949116934 | - |
dc.identifier.wosid | 000347135600017 | - |
dc.identifier.bibliographicCitation | BIOELECTROCHEMISTRY, v.101, pp.114 - 119 | - |
dc.relation.isPartOf | BIOELECTROCHEMISTRY | - |
dc.citation.title | BIOELECTROCHEMISTRY | - |
dc.citation.volume | 101 | - |
dc.citation.startPage | 114 | - |
dc.citation.endPage | 119 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.subject.keywordPlus | BIOFUEL CELLS | - |
dc.subject.keywordPlus | CARBON | - |
dc.subject.keywordPlus | CHALLENGES | - |
dc.subject.keywordAuthor | Enzymatic glucose sensors | - |
dc.subject.keywordAuthor | Glucose oxidase | - |
dc.subject.keywordAuthor | Carbon nanotubes | - |
dc.subject.keywordAuthor | Electron generation and transfer | - |
dc.subject.keywordAuthor | Enzyme precipitate coating | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1567539414001339?via%3Dihub | - |
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