Eugenol Inhibits the GABA(A) Current in Trigeminal Ganglion Neurons
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sang Hoon | - |
dc.contributor.author | Moon, Jee Youn | - |
dc.contributor.author | Jung, Sung Jun | - |
dc.contributor.author | Kang, Jin Gu | - |
dc.contributor.author | Choi, Seung Pyo | - |
dc.contributor.author | Jang, Jun Ho | - |
dc.date.accessioned | 2022-07-16T01:04:58Z | - |
dc.date.available | 2022-07-16T01:04:58Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158189 | - |
dc.description.abstract | Eugenol has sedative, antioxidant, anti-inflammatory, and analgesic effects, but also serves as an irritant through the regulation of a different set of ion channels. Activation of gamma aminobutyric acid (GABA) receptors on sensory neurons leads to the stabilization of neuronal excitability but contributes to formalin-induced inflammatory pain. In this study, we examined the effect of eugenol on the GABA-induced current in rat trigeminal ganglia (TG) neurons and in human embryonic kidney (HEK) 293 cells expressing the GABA(A) receptor alpha 1 beta 2 gamma 2 subtype using the whole-cell patch clamp technique. RT-PCR and Western blot analysis were used to confirm the expression of GABA(A) receptor gamma 2 subunit mRNA and protein in the TG and hippocampus. Eugenol decreased the amplitude ratio of the GABA-induced current to 27.5 +/- 3.2% (p < 0.05) in TG neurons, which recovered after a 3-min washout. In HEK 293 cells expressing the alpha 1 beta 2.2 subtype, eugenol inhibited GABA-induced currents in a dose-dependent manner. Application of eugenol also decreased the GABA response in the presence of a G-protein blocker. Eugenol pretreatment with different concentrations of GABA resulted in similar inhibition of the GABA-induced current in a noncompetitive manner. In conclusion, eugenol inhibits the GABA-induced current in TG neurons and HEK 293 cells expressing the GABA(A) receptor in a reversible, dose-dependent, and non-competitive manner, but not via the G-protein pathway. We suggest that the GABA(A) receptor could be a molecular target for eugenol in the modulation of nociceptive information. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Eugenol Inhibits the GABA(A) Current in Trigeminal Ganglion Neurons | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jung, Sung Jun | - |
dc.identifier.doi | 10.1371/journal.pone.0117316 | - |
dc.identifier.scopusid | 2-s2.0-84922174504 | - |
dc.identifier.wosid | 000350680700080 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.10, no.1, pp.1 - 12 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | SPINAL-CORD | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | SUBUNIT | - |
dc.subject.keywordPlus | SUPERFAMILY | - |
dc.identifier.url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0117316 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.