Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice
DC Field | Value | Language |
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dc.contributor.author | Kim, Mi Na | - |
dc.contributor.author | Koh, Seong-Joon | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Im, Jong Pil | - |
dc.contributor.author | Jung, Hyun Chae | - |
dc.contributor.author | Kim, Joo Sung | - |
dc.date.accessioned | 2022-07-16T01:39:03Z | - |
dc.date.available | 2022-07-16T01:39:03Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 1007-9327 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158498 | - |
dc.description.abstract | AIM: To investigate the effect of Clostridium difficile (C. difficile) infection in an interleukin 10-deficient (IL-10(-/-)) mouse model of inflammatory bowel disease. METHODS: Bone marrow-derived dendritic cells isolated from wild type (WT) and IL-10(-/-) mice were stimulated for 4 h with C. difficile toxin A (200 mu g/mL), and gene expression of interferon (IFN)-gamma, IL-12 and IL-23 was determined by real-time reverse transcription polymerase chain reaction. WT and IL-10(-/-) mice (n = 20 each) were exposed to an antibiotic cocktail for three days and then were injected with clindamycin (i.p.). Mice (n = 10 WT, 10 IL-10(-/-)) were then challenged with oral administration of C. difficile (1 x 10(5) colony forming units of strain VPI 10463). Animals were monitored daily for 7 d for signs of colitis. Colonic tissue samples were evaluated for cytokine gene expression and histopathologic analysis. RESULTS: C. difficile toxin A treatment induced IFN-gamma gene expression to a level that was significantly higher in BDMCs from IL-10(-/-) compared to those from WT mice (P < 0.05). However, expression of IL-12 and IL-23 was not different among the groups. Following C. difficile administration, mice developed diarrhea and lost weight within 2-3 d. Weight loss was significantly greater in IL-10(-/-) compared to WT mice (P < 0.05). C. difficile infection induced histopathologic features typical of colitis in both IL-10(-/-) and WT mice. The histopathologic severity score was significantly higher in the IL-10(-/)- than in WT mice (mean +/- standard error; 5.50 +/- 0.53 vs 2.44 +/- 0.46; P < 0.05). This was accompanied by a significantly greater increase in IFN-gamma gene expression in colonic tissues from IL-10(-/-) than from WT mice challenged with C. difficile (P < 0.05). CONCLUSION: These results indicate that colitis is more severe after C. difficile infection in IL-10(-/-) mice, and that IFN-gamma expression is involved in this process. (C) 2014 Baishideng Publishing Group Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BAISHIDENG PUBLISHING GROUP INC | - |
dc.title | Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.identifier.doi | 10.3748/wjg.v20.i45.17084 | - |
dc.identifier.scopusid | 2-s2.0-84917706337 | - |
dc.identifier.wosid | 000346050700027 | - |
dc.identifier.bibliographicCitation | WORLD JOURNAL OF GASTROENTEROLOGY, v.20, no.45, pp.17084 - 17091 | - |
dc.relation.isPartOf | WORLD JOURNAL OF GASTROENTEROLOGY | - |
dc.citation.title | WORLD JOURNAL OF GASTROENTEROLOGY | - |
dc.citation.volume | 20 | - |
dc.citation.number | 45 | - |
dc.citation.startPage | 17084 | - |
dc.citation.endPage | 17091 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | INFLAMMATORY-BOWEL-DISEASE | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | IFN-GAMMA | - |
dc.subject.keywordPlus | INTERLEUKIN-10-DEFICIENT MICE | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | IL-10-DEFICIENT MICE | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | TOXIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ENTEROCOLITIS | - |
dc.subject.keywordAuthor | Clostridium difficile | - |
dc.subject.keywordAuthor | Inflammatory bowel disease | - |
dc.subject.keywordAuthor | Interleukin 10-deficient mice | - |
dc.subject.keywordAuthor | Interferon-gamma | - |
dc.subject.keywordAuthor | Colitis | - |
dc.identifier.url | https://www.wjgnet.com/1007-9327/full/v20/i45/17084.htm | - |
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