Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice

Full metadata record
DC Field Value Language
dc.contributor.authorKim, Mi Na-
dc.contributor.authorKoh, Seong-Joon-
dc.contributor.authorKim, Jung Mogg-
dc.contributor.authorIm, Jong Pil-
dc.contributor.authorJung, Hyun Chae-
dc.contributor.authorKim, Joo Sung-
dc.date.accessioned2022-07-16T01:39:03Z-
dc.date.available2022-07-16T01:39:03Z-
dc.date.created2021-05-12-
dc.date.issued2014-12-
dc.identifier.issn1007-9327-
dc.identifier.urihttps://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158498-
dc.description.abstractAIM: To investigate the effect of Clostridium difficile (C. difficile) infection in an interleukin 10-deficient (IL-10(-/-)) mouse model of inflammatory bowel disease. METHODS: Bone marrow-derived dendritic cells isolated from wild type (WT) and IL-10(-/-) mice were stimulated for 4 h with C. difficile toxin A (200 mu g/mL), and gene expression of interferon (IFN)-gamma, IL-12 and IL-23 was determined by real-time reverse transcription polymerase chain reaction. WT and IL-10(-/-) mice (n = 20 each) were exposed to an antibiotic cocktail for three days and then were injected with clindamycin (i.p.). Mice (n = 10 WT, 10 IL-10(-/-)) were then challenged with oral administration of C. difficile (1 x 10(5) colony forming units of strain VPI 10463). Animals were monitored daily for 7 d for signs of colitis. Colonic tissue samples were evaluated for cytokine gene expression and histopathologic analysis. RESULTS: C. difficile toxin A treatment induced IFN-gamma gene expression to a level that was significantly higher in BDMCs from IL-10(-/-) compared to those from WT mice (P < 0.05). However, expression of IL-12 and IL-23 was not different among the groups. Following C. difficile administration, mice developed diarrhea and lost weight within 2-3 d. Weight loss was significantly greater in IL-10(-/-) compared to WT mice (P < 0.05). C. difficile infection induced histopathologic features typical of colitis in both IL-10(-/-) and WT mice. The histopathologic severity score was significantly higher in the IL-10(-/)- than in WT mice (mean +/- standard error; 5.50 +/- 0.53 vs 2.44 +/- 0.46; P < 0.05). This was accompanied by a significantly greater increase in IFN-gamma gene expression in colonic tissues from IL-10(-/-) than from WT mice challenged with C. difficile (P < 0.05). CONCLUSION: These results indicate that colitis is more severe after C. difficile infection in IL-10(-/-) mice, and that IFN-gamma expression is involved in this process. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.-
dc.language영어-
dc.language.isoen-
dc.publisherBAISHIDENG PUBLISHING GROUP INC-
dc.titleClostridium difficile infection aggravates colitis in interleukin 10-deficient mice-
dc.typeArticle-
dc.contributor.affiliatedAuthorKim, Jung Mogg-
dc.identifier.doi10.3748/wjg.v20.i45.17084-
dc.identifier.scopusid2-s2.0-84917706337-
dc.identifier.wosid000346050700027-
dc.identifier.bibliographicCitationWORLD JOURNAL OF GASTROENTEROLOGY, v.20, no.45, pp.17084 - 17091-
dc.relation.isPartOfWORLD JOURNAL OF GASTROENTEROLOGY-
dc.citation.titleWORLD JOURNAL OF GASTROENTEROLOGY-
dc.citation.volume20-
dc.citation.number45-
dc.citation.startPage17084-
dc.citation.endPage17091-
dc.type.rimsART-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaGastroenterology & Hepatology-
dc.relation.journalWebOfScienceCategoryGastroenterology & Hepatology-
dc.subject.keywordPlusINFLAMMATORY-BOWEL-DISEASE-
dc.subject.keywordPlusDENDRITIC CELLS-
dc.subject.keywordPlusIFN-GAMMA-
dc.subject.keywordPlusINTERLEUKIN-10-DEFICIENT MICE-
dc.subject.keywordPlusINTERFERON-GAMMA-
dc.subject.keywordPlusIL-10-DEFICIENT MICE-
dc.subject.keywordPlusPATHOGENESIS-
dc.subject.keywordPlusTOXIN-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusENTEROCOLITIS-
dc.subject.keywordAuthorClostridium difficile-
dc.subject.keywordAuthorInflammatory bowel disease-
dc.subject.keywordAuthorInterleukin 10-deficient mice-
dc.subject.keywordAuthorInterferon-gamma-
dc.subject.keywordAuthorColitis-
dc.identifier.urlhttps://www.wjgnet.com/1007-9327/full/v20/i45/17084.htm-
Files in This Item
Go to Link
Appears in
Collections
서울 의과대학 > 서울 미생물학교실 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kim, Jung Mogg photo

Kim, Jung Mogg
COLLEGE OF MEDICINE (DEPARTMENT OF MEDICAL MICROBIOLOGY)
Read more

Altmetrics

Total Views & Downloads

BROWSE