Wilms' tumor suppressor gene (WT1) suppresses apoptosis by transcriptionally downregulating BAX expression in immature rat granulosa cells
DC Field | Value | Language |
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dc.contributor.author | Park, Minji | - |
dc.contributor.author | Choi, Yuri | - |
dc.contributor.author | Choi, Hyeonhae | - |
dc.contributor.author | Roh, Jaesook | - |
dc.date.accessioned | 2022-07-16T01:39:22Z | - |
dc.date.available | 2022-07-16T01:39:22Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.issn | 1757-2215 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158502 | - |
dc.description.abstract | Background: The important role of WT1 in early folliculogenesis was evident from its restricted expression pattern in immature follicles and from its involvement in transcriptional control of inhibin-a and FSH receptor. There is also considerable evidence that WT1 is a potent inhibitor of apoptotic cell death in the developing kidney and male germ cells, suggesting that it could play a role in the regulation of follicle survival. Therefore, we evaluated if WT1 involves in cell survival of granulosa cells (GCs) during the FSH-independent stage. Methods: GCs were obtained from small preantral follicles of immature rat ovary. Bax and bcl-2 mRNA and protein levels in GCs transfected with WT1 (-KTS) or WT1 (+KTS) were analyzed by Real-time RT-PCR and immune-blotting analysis. Cell viability was measured with MTT assays and apoptosis was analyzed with caspase 3/7 activity and TUNEL assay. The mechanism by which WT1 regulates Bax expression was investigated using Bax promoter-luciferase reporter assay and ChIP assays from GCs. Results: Here, we showed that WT1 (-KTS) suppressed endogenous Bax transcript and protein expression, and this inhibition resulted from direct binding of WT1 in the Bax promoter region in vivo. In addition, anti-apoptotic effects of WT1 (-KTS) were demonstrated based on MTT assays, a sensitive bioluminescence caspase 3/7 assay and TUNEL assays. On the other hand, WT1 has no role on bcl-2 expression in GCs. Conclusion: These findings suggest that activation of WT1 is necessary for maintenance of GC survival during early stage of follicles and WT1 can play a role in protecting apoptosis through the regulation of upstream activator (Bax), as well as through regulation of downstream effecter (caspases 3 and 7). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Wilms' tumor suppressor gene (WT1) suppresses apoptosis by transcriptionally downregulating BAX expression in immature rat granulosa cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Roh, Jaesook | - |
dc.identifier.doi | 10.1186/s13048-014-0118-y | - |
dc.identifier.scopusid | 2-s2.0-84964696677 | - |
dc.identifier.wosid | 000347193000004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF OVARIAN RESEARCH, v.7, pp.1 - 10 | - |
dc.relation.isPartOf | JOURNAL OF OVARIAN RESEARCH | - |
dc.citation.title | JOURNAL OF OVARIAN RESEARCH | - |
dc.citation.volume | 7 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.subject.keywordPlus | MESSENGER-RIBONUCLEIC-ACID | - |
dc.subject.keywordPlus | FOLLICULAR ATRESIA | - |
dc.subject.keywordPlus | OVARIAN-FOLLICLES | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | BCL-2 | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | PRODUCT | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordAuthor | WT1 | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Bax | - |
dc.subject.keywordAuthor | bcl-2 | - |
dc.subject.keywordAuthor | Caspase | - |
dc.subject.keywordAuthor | Granulosa cell | - |
dc.subject.keywordAuthor | Rat | - |
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