Enhanced gene disruption by programmable nucleases delivered by a minicircle vector
DC Field | Value | Language |
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dc.contributor.author | Dad, A-B K. | - |
dc.contributor.author | Ramakrishna, S. | - |
dc.contributor.author | Song, M. | - |
dc.contributor.author | Kim, H. | - |
dc.date.accessioned | 2022-07-16T02:04:03Z | - |
dc.date.available | 2022-07-16T02:04:03Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-11 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158724 | - |
dc.description.abstract | Targeted genetic modification using programmable nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) is of great value in biomedical research, medicine and biotechnology. Minicircle vectors,which lack extraneous bacterial sequences, have several advantages over conventional plasmids for transgene delivery. Here, for the first time, we delivered programmable nucleases into human cells using transient,transfection of a minicircle vector and compared the results with those obtained using a conventional plasmid. Surrogate reporter assays and T7 endonuclease analyses revealed that cells in the minicircle vector group displayed significantly higher mutation frequencies at the target sites than those in the conventional plasmid group. Quantitative PCR and reverse transcription-PCR showed higher vector copy number and programmable nuclease transcript levels, respectively, in 293T cells after minicircle versus conventional plasmid vector transfection. In addition, tryphan blue staining and flow cytometry after annexin V and propidium iodide staining showed that cell viability was also significantly higher in the minicircle group than in the conventional plasmid group. Taken together, our results show that gene disruption using minicircle vector-mediated delivery of ZFNs and TALENs is a more efficient, safer and less toxic method than using a conventional plasmid, and indicate that the minicircle vector could serve as an advanced delivery method for programmable nucleases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Enhanced gene disruption by programmable nucleases delivered by a minicircle vector | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ramakrishna, S. | - |
dc.identifier.doi | 10.1038/gt.2014.76 | - |
dc.identifier.scopusid | 2-s2.0-84909988097 | - |
dc.identifier.wosid | 000344835700001 | - |
dc.identifier.bibliographicCitation | GENE THERAPY, v.21, no.11, pp.921 - 930 | - |
dc.relation.isPartOf | GENE THERAPY | - |
dc.citation.title | GENE THERAPY | - |
dc.citation.volume | 21 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 921 | - |
dc.citation.endPage | 930 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | ZINC-FINGER NUCLEASES | - |
dc.subject.keywordPlus | HOMOLOGOUS RECOMBINATION | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | EMBRYO MICROINJECTION | - |
dc.subject.keywordPlus | GENOME MODIFICATION | - |
dc.subject.keywordPlus | KNOCKOUT RATS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | TALEN | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | ENRICHMENT | - |
dc.identifier.url | https://www.nature.com/articles/gt201476 | - |
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