Transient Freezing Behavior in Photophobic Responses of Euglena gracilis Investigated in a Microfluidic Device
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ozasa, Kazunari | - |
dc.contributor.author | Lee, Jeesoo | - |
dc.contributor.author | Song, Simon | - |
dc.contributor.author | Maeda, Mizuo | - |
dc.date.accessioned | 2022-07-16T02:42:12Z | - |
dc.date.available | 2022-07-16T02:42:12Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-10 | - |
dc.identifier.issn | 0032-0781 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158989 | - |
dc.description.abstract | We found that the transient freezing behavior in photophobic responses of Euglena gracilis is a good indicator of the metabolic status of the cells. The transient blue light photophobic responses of E. gracilis cells were investigated on-chip using a new measurement, 'trace momentum' (TM), to evaluate their swimming activity quantitatively in real time. When blue light of intensity >30 mW cm(-2) was repeatedly switched on and off, a large negative spike in the TM was observed at the onset of the 'blue-light-off' phase. Single-cell trace analysis at a blue light intensity of 40 mW cm(-2) showed that 48% (on average, n = 15) of tumbling Euglena cells ceased activity ('freezing') for 2-30 s at the onset of blue-light-off before commencing forward motion in a straight line (termed 'straight forward swimming'), while 45% smoothly commenced straightforward swimming without delay. The proportion of freezing Euglena cells depended on the blue light intensity (only 20% at 20 mW cm(-2)). When the cells were stimulated by four blue light pulses at the higher intensity, without pre-exposure, the transient freezing behavior was more prominent but, on repeating the stimuli after an 80 min interval in red light, the same cells did not freeze. This shows that the metabolism of the cells had changed to anti-freezing during the interval. The relationship between the interval time with/without light irradiation and the blue light adaptation was elucidated experimentally. The origin of the freezing behavior is considered to be a shortage of a metabolic substance that promotes smooth switching of flagellum movement from in situ rotation mode to a straightforward swimming mode. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Transient Freezing Behavior in Photophobic Responses of Euglena gracilis Investigated in a Microfluidic Device | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Song, Simon | - |
dc.identifier.doi | 10.1093/pcp/pcu101 | - |
dc.identifier.scopusid | 2-s2.0-84937947015 | - |
dc.identifier.wosid | 000342978800002 | - |
dc.identifier.bibliographicCitation | PLANT AND CELL PHYSIOLOGY, v.55, no.10, pp.1704 - 1712 | - |
dc.relation.isPartOf | PLANT AND CELL PHYSIOLOGY | - |
dc.citation.title | PLANT AND CELL PHYSIOLOGY | - |
dc.citation.volume | 55 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1704 | - |
dc.citation.endPage | 1712 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | ADENYLYL-CYCLASE PAC | - |
dc.subject.keywordPlus | ACTION SPECTRA | - |
dc.subject.keywordPlus | STEP-UP | - |
dc.subject.keywordPlus | CELL-CYCLE | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | PHOTOTAXIS | - |
dc.subject.keywordPlus | FLAGELLATE | - |
dc.subject.keywordPlus | BIODIESEL | - |
dc.subject.keywordPlus | PHOTOMOVEMENTS | - |
dc.subject.keywordPlus | PHOTOKINESIS | - |
dc.subject.keywordAuthor | Adaptation | - |
dc.subject.keywordAuthor | Blue light | - |
dc.subject.keywordAuthor | Euglena gracilis | - |
dc.subject.keywordAuthor | Microfluidic aquarium | - |
dc.subject.keywordAuthor | Photophobic response | - |
dc.subject.keywordAuthor | Transient responses | - |
dc.identifier.url | https://academic.oup.com/pcp/article/55/10/1704/2755956 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.