Membrane-embedded disposable microfluidic chip for cardiac biomarker screening

Abstract

We report a simple and multifunctional microfluidic cartridge with electrochemical (EC) sensing for cardiac biomarker screening including a passive valve, reagent storage and electrochemical sensors from a layer-by-layer rapid prototyping technique. By embedding multiple membranes in the device, immunoassay procedures were performed by controlling fluidic paths and releasing reagents to obtain proper reactions on the electrodes. With the disposable multifunctional cartridge device, we was able to detect as low as 0.25 ng/mL of Creatine Kinase (CK)-Myocardial Band (MB) automatically.