Enrichment of cells with TALEN-induced mutations using surrogate reporters
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Young-Hoon | - |
dc.contributor.author | Ramakrishna, Suresh | - |
dc.contributor.author | Kim, Hyongbum | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2022-07-16T03:43:20Z | - |
dc.date.available | 2022-07-16T03:43:20Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-08 | - |
dc.identifier.issn | 1046-2023 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/159441 | - |
dc.description.abstract | Targeted gene knockout using engineered nucleases such as transcription activator like-effector nucleases (TALENs) is a gold standard for investigating the functions of a gene of interest. Although most TALENs can cleave chromosomal DNA efficiently, the activities of designed TALENs are not always high enough to allow the efficient derivation of cells containing TALEN-driven mutations. Thus, simple methods to enrich cells containing TALEN-directed mutations would facilitate the use of TALENs. Here we describe the enrichment of such cells using surrogate episomal reporters coupled with flow cytometric sorting, magnetic separation, or hygromycin selection. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Enrichment of cells with TALEN-induced mutations using surrogate reporters | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ramakrishna, Suresh | - |
dc.identifier.doi | 10.1016/j.ymeth.2014.04.012 | - |
dc.identifier.scopusid | 2-s2.0-84929514924 | - |
dc.identifier.wosid | 000341803500015 | - |
dc.identifier.bibliographicCitation | METHODS, v.69, no.1, pp.108 - 117 | - |
dc.relation.isPartOf | METHODS | - |
dc.citation.title | METHODS | - |
dc.citation.volume | 69 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 108 | - |
dc.citation.endPage | 117 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | MAJOR HISTOCOMPATIBILITY COMPLEX | - |
dc.subject.keywordPlus | HYGROMYCIN-B PHOSPHOTRANSFERASE | - |
dc.subject.keywordPlus | GENE KNOCKOUT | - |
dc.subject.keywordPlus | BETA-2-MICROGLOBULIN | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | TRANSLOCATION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordAuthor | Transcription activator-like effector nucleases | - |
dc.subject.keywordAuthor | Enrichment | - |
dc.subject.keywordAuthor | Mutant cells | - |
dc.subject.keywordAuthor | Flow cytometry | - |
dc.subject.keywordAuthor | Magnetic separation | - |
dc.subject.keywordAuthor | Hygromycin B | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1046202314001650?via%3Dihub | - |
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