Anti-inflammatory mechanism of metformin and its effects in intestinal inflammation and colitis-associated colon cancer
DC Field | Value | Language |
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dc.contributor.author | Koh, Seong-Joon | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Kim, In-Kyoung | - |
dc.contributor.author | Ko, Su Hyuk | - |
dc.contributor.author | Kim, Joo Sung | - |
dc.date.accessioned | 2022-07-16T05:41:44Z | - |
dc.date.available | 2022-07-16T05:41:44Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-03 | - |
dc.identifier.issn | 0815-9319 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/160498 | - |
dc.description.abstract | Background and AimThe aim of this study is to evaluate the effect of metformin on intestinal inflammation. MethodsCOLO205 cells were pretreated with metformin and stimulated with tumor necrosis factor (TNF)-. Expression of interleukin (IL)-8 was determined by luciferase assay and real-time PCR. Inhibitor of kappaB (IB) phosphorylation/degradation and adenosine monohosphate-activated protein kinase (AMPK) activity were evaluated by Western blotting. DNA-binding activity of transcription factor nuclear factor-kappaB (NF-B) was assessed by electrophoretic mobility shift assay. In an acute colitis model, mice were given 4% dextran sulfate sodium (DSS) for 5 days. IL-10(-/-) mice were used to evaluate the effect of metformin on chronic colitis. In an inflamation-associated tumor model, mice were given a single intraperitoneal injection of azoxymethane followed by three cycles of 2% DSS for 5 days and 2 weeks of free water consumption. ResultsMetformin significantly inhibited IL-8 induction in COLO 205 cells stimulated with TNF-. Metformin attenuated IB phosphorylation and NF-B DNA-binding activity. Administration of metformin significantly reduced the severity of DSS-induced colitis. In addition, DSS-induced IB kinase (IKK) activation was significantly reduced in mice treated with metformin. Metformin significantly attenuated the severity of colitis in IL-10(-/-) mice, induced AMPK activity in intestinal epithelial cells, and inhibited the development of colitic cancer in mice. ConclusionsThese results indicate that metformin suppresses NF-B activation in intestinal epithelial cells and ameliorates murine colitis and colitis-associated tumorigenesis in mice, suggesting that metformin could be a potential therapeutic agent for the treatment of inflammatory bowel disease. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Anti-inflammatory mechanism of metformin and its effects in intestinal inflammation and colitis-associated colon cancer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.identifier.doi | 10.1111/jgh.12435 | - |
dc.identifier.scopusid | 2-s2.0-84897643844 | - |
dc.identifier.wosid | 000331469400017 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, v.29, no.3, pp.502 - 510 | - |
dc.relation.isPartOf | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.title | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.volume | 29 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 502 | - |
dc.citation.endPage | 510 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | ACUTE MURINE COLITIS | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL ACTIVITY | - |
dc.subject.keywordPlus | BOWEL-DISEASE | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordAuthor | colitis | - |
dc.subject.keywordAuthor | inflammatory bowel disease | - |
dc.subject.keywordAuthor | metformin | - |
dc.subject.keywordAuthor | NF-kappa B | - |
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